Human parvovirus B19 VP1u Protein as inflammatory mediators induces liver injury in naïve mice

Autor:	Tzy-Yen Chen, Bor-Show Tzang, Hsu-Chin Chan, Ya-Fang Shi, Shun-Chih Chang, Chun-Ching Chiu, Tsai-Ching Hsu
Rok vydání:	2016
Předmět:	0301 basic medicine Microbiology (medical) Intravital Microscopy viruses Interleukin-1beta 030106 microbiology Immunology Inflammation Biology Transfection Microbiology Pathogenesis Mice 03 medical and health sciences In vivo hemic and lymphatic diseases Chlorocebus aethiops Parvovirus B19 Human medicine Animals Zymography Phosphorylation STAT3 Liver injury Mice Inbred BALB C Interleukin-6 virus diseases medicine.disease 030104 developmental biology Infectious Diseases medicine.anatomical_structure Liver Hepatitis Viral Animal Hepatocyte COS Cells biology.protein Capsid Proteins Parasitology Inflammation Mediators medicine.symptom Research Paper
Zdroj:	Virulence. 7:110-118
ISSN:	2150-5608 2150-5594
Popis:	Human parvovirus B19 (B19V) is a human pathogen known to be associated with many non-erythroid diseases, including hepatitis. Although B19V VP1-unique region (B19-VP1u) has crucial roles in the pathogenesis of B19V infection, the influence of B19-VP1u proteins on hepatic injury is still obscure. This study investigated the effect and possible inflammatory signaling of B19-VP1u in livers from BALB/c mice that were subcutaneously inoculated with VP1u-expressing COS-7 cells. The in vivo effects of B19-VP1u were analyzed by using live animal imaging system (IVIS), Haematoxylin-Eosin staining, gel zymography, and immunoblotting after inoculation. Markedly hepatocyte disarray and lymphocyte infiltration, enhanced matrix metalloproteinase (MMP)-9 activity and increased phosphorylation of p38, ERK, IKK-α, IκB and NF-κB (p-p65) proteins were observed in livers from BALB/c mice receiving COS-7 cells expressing B19-VP1u as well as the significantly increased CRP, IL-1β and IL-6. Notably, IFN-γ and phosphorylated STAT1, but not STAT3, were also significantly increased in the livers of BALB/c mice that were subcutaneously inoculated with VP1u-expressing COS-7 cells. These findings revealed the effects of B19-VP1u on liver injury and suggested that B19-VP1u may have a role as mediators of inflammation in B19V infection.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66b9441a731867c9b0534e47c1af5b99 https://doi.org/10.1080/21505594.2015.1122165 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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