Characterization of two unusual clinically significant Francisella strains
| Autor: | A. Sjösted, Lan Vo, Gunnar Sandström, Rabih O. Darouiche, Paul R. Georghiou, Ribhi M. Shawar, Jill E. Clarridge, C. Osting, Teresa J. Raich |
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| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Adult Male Biovar Molecular Sequence Data Bacteremia Microbial Sensitivity Tests DNA Ribosomal Polymerase Chain Reaction Microbiology chemistry.chemical_compound Chocolate agar Agglutination Tests Pneumonia Bacterial Humans Trypticase soy agar Francisella tularensis Antiserum biology Hybridization probe Fatty Acids Middle Aged biology.organism_classification Virology Bacterial Typing Techniques Culture Media chemistry RNA Ribosomal Francisella Bacteria Polymorphism Restriction Fragment Length Research Article |
| Zdroj: | Journal of clinical microbiology. 34(8) |
| ISSN: | 0095-1137 |
| Popis: | We have isolated two phenotypically distinct nonfastidious Francisella strains (Fx1 and Fx2) from the blood of compromised patients with pneumonia and compared them with eight other Francisella strains, including Francisella tularensis biovar tularensis, F. tularensis biovar novicida, and F. philomiragia. Our isolates grew well on sheep blood agar, chocolate agar, modified Thayer-Martin agar, and Trypticase soy agar. Fx1 and Fx2 were determined to be within the Francisella genus by cellular fatty acid analysis and by the utilization of glucose, production of H2S and catalase, and lack of motility, oxidase, nitrate reductase, and gelatinase. They were additionally shown to belong to the species F. tularensis by sequencing of two variable regions comprising approximately 500 nucleotides of the 16S rRNA gene. Also, RNA probe hybridization confirmed their belonging to the species F. tularensis. However, the new strains, which are not identical, are distinguished from other F. tularensis strains by growth characteristics, repetitive extragenic palindromic PCR fragment pattern, and some biochemical tests. Key biochemical differences included the findings that Fx1 was positive for beta-galactosidase and arabinose hydrolysis and that both strains were citrulline ureidase positive and glycerol negative. Commercial F. tularensis antiserum agglutinated stock F. tularensis strains but not Fx1, Fx2, F. tularensis biovar novicida, or F. philomiragia; serum from either patient failed to agglutinate or only weakly agglutinated commercial antigen but showed agglutination when tested against each patient's respective isolate. Fx1 and Fx2 produced beta-lactamase. Because of their good growth, negative serology, and biochemical profile, the organisms could be misidentified in the clinical laboratory if standard strategies or commercial identification systems are used. |
| Databáze: | OpenAIRE |
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