The use of the preparation of F(ab')2 antibody from soluble immune complexes to determine the complexed antigens
| Autor: | Jill Burton-Kee, J.F. Mowbray, Colette Dambuyant |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Immunology
Pepsin digestion Polyethylene glycol Antigen-Antibody Complex chemistry.chemical_compound Immunoglobulin Fab Fragments Immune system Antigen Antibody Specificity PEG ratio Immunology and Allergy Animals Humans Antigens Immunoelectrophoresis biology Chemistry Experimental model Complement C3 Molecular biology Pepsin A Biochemistry Solubility Reagent biology.protein Rabbits Antibody Filtration |
| Zdroj: | Journal of immunological methods. 24(1-2) |
| ISSN: | 0022-1759 |
| Popis: | An autoradiographic technique for the characterization of antibody specificity in soluble antigen—antibody complexes has been developed. The circulating antigen—antibody complexes are precipitated by polyethylene glycol (PEG). The antibodies are liberated as F(ab′) 2 from the complexes by pepsin digestion. The antibody specificity against the putative antigen was revealed by radioimmunoelectrophoresis using [ 125 I]-F(ab′) 2 reagents. The method was developed by using an experimental model of C3/anti-C3 complexes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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