Oxygen Plasma Technology-Assisted Preparation of Three-Dimensional Reduced Graphene Oxide/Polypyrrole/Strontium Composite Scaffold for Repair of Bone Defects Caused by Osteoporosis
| Autor: | Zebiao Kang, Na Wang, Xiaoxue Mai, Weibo Xie, Xiaoli Qin, Fuxiang Song |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
oxygen plasma technology
Scaffold Materials science Biocompatibility Polymers Osteoporosis Oxide Pharmaceutical Science Organic chemistry Biocompatible Materials 02 engineering and technology scaffold 010402 general chemistry Polypyrrole 01 natural sciences Article Analytical Chemistry law.invention Cell Line chemistry.chemical_compound Mice QD241-441 Tissue engineering law Drug Discovery medicine Animals MTT assay Pyrroles Physical and Theoretical Chemistry strontium (Sr) bone defect Osteoblasts Tissue Scaffolds Graphene 021001 nanoscience & nanotechnology medicine.disease three-dimensional (3D) 0104 chemical sciences chemistry Chemistry (miscellaneous) Strontium tissue engineering Printing Three-Dimensional Molecular Medicine Graphite 0210 nano-technology Biomedical engineering |
| Zdroj: | Molecules, Vol 26, Iss 4451, p 4451 (2021) Molecules Volume 26 Issue 15 |
| ISSN: | 1420-3049 |
| Popis: | Repairs of bone defects caused by osteoporosis have always relied on bone tissue engineering. However, the preparation of composite tissue engineering scaffolds with a three-dimensional (3D) macroporous structure poses huge challenges in achieving osteoconduction and osteoinduction for repairing bone defects caused by osteoporosis. In the current study, a three-dimensional macroporous (150–300 μm) reduced graphene oxide/polypyrrole composite scaffold modified by strontium (Sr) (3D rGO/PPY/Sr) was successfully prepared using the oxygen plasma technology-assisted method, which is simple, safe, and inexpensive. The findings of the MTT assay and AO/EB fluorescence double staining showed that 3D rGO/PPY/Sr has a good biocompatibility and effectively promoted MC3T3-E1 cell proliferation. Furthermore, the ALP assay and alizarin red staining showed that 3D rGO/PPY/Sr increased the expression levels of ALP activity and the formation of calcified nodules. The desirable biocompatibility, osteoconduction, and osteoinduction abilities, assure that the 3D macroporous rGO/PPY/Sr composite scaffold offers promising potential for use in the repair of bone defects caused by osteoporosis in bone tissue engineering. |
| Databáze: | OpenAIRE |
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