Crosstalk between miRNAs and their regulated genes network in stroke
| Autor: | Ping Wu, Jun Liu, Jie Wang, Yanan Yu, Yingying Zhang, Ruixia Kang, Zhong Wang, ChunFeng Shen, Chun-Ti Shen, Ye Yuan |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Gene regulatory network Computational biology Disease Bioinformatics Article 03 medical and health sciences 0302 clinical medicine RNA interference microRNA Databases Genetic Medicine Humans Gene Regulatory Networks RNA Messenger Gene Regulation of gene expression Multidisciplinary business.industry Gene Expression Profiling Computational Biology Reproducibility of Results Molecular Sequence Annotation Gene expression profiling Stroke Crosstalk (biology) MicroRNAs 030104 developmental biology Gene Ontology Gene Expression Regulation RNA Interference business 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | In recent years, more and more studies focus on the roles of genes or miRNAs in stroke. However, the molecular mechanism connecting miRNAs and their targetgenes remains unclear. The aim of this study was to determine the differential regulation and correlations between miRNAs and their targetgenes in human stroke. Stroke-related miRNAs were obtained from the Human MicroRNA Disease Database (HMDD) and their targetgenes were generated from three independent sources. Kappa score was used to create the network and the functional modules. A total of 11 stroke-related miRNAs were identified from the HMDD and 441 overlapping targetgenes were extracted from the three databases. By network construction and GO analysis, 13 functional modules, 186 biological processes and 21 pathways were found in the network, of which functional module 8 was the largest module, cellular-related process and phosphate-related process were the most important biological processes and MAPK signaling pathway was the most significant pathway. In our study, all miRNAs regulate the stroke modular network by their targetgenes. After the validation of miRNAs, we found that miR-605 and miR-181d were highly expressed in the blood of stroke patients which never reported before may supply novel target for treatment. |
| Databáze: | OpenAIRE |
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