Isolation and Replication of Rabies Virus in C6 Rat Glioma Cells (Clone CCL-107)
| Autor: | Juliano Bordignon, Maria Luiza Carrieri, A. T. Piza, Carlos R. Zanetti, G. M. M. Caporale, Ivanete Kotait, M. Alvarez-Silva |
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| Rok vydání: | 2001 |
| Předmět: |
Virus Cultivation
viruses Cell Clone (cell biology) Bioengineering Biology Virus Replication medicine.disease_cause Applied Microbiology and Biotechnology Cell Line Mice Viral Envelope Proteins Cricetinae Tumor Cells Cultured medicine Animals Nucleocapsid Antigens Viral Direct fluorescent antibody Glycoproteins Pharmacology chemistry.chemical_classification General Immunology and Microbiology Strain (chemistry) Inoculation Rabies virus Glioma General Medicine Nucleocapsid Proteins medicine.disease Virology Rats medicine.anatomical_structure chemistry Fluorescent Antibody Technique Direct Cattle Rabies Glycoprotein Biotechnology |
| Zdroj: | Biologicals. 29:67-73 |
| ISSN: | 1045-1056 |
| Popis: | The susceptibility of the C6 rat glioma cell line (ATCC; CCL-107) to rabies virus was characterized. The kinetics of infection performed with a fixed and a wild strain (from an infected cow) of rabies virus was monitored by direct immunofluorescence. Fluorescent cytoplasmic bodies were readily observed by UV microscopy from 24 hours post-infection (hpi) onwards. The ability of C6 to produce rabies infective virion particles was confirmed by determining the viral titres present in the supernatants of infected cultures, by both BHK-21 cell infection and mice inoculation. C6 cells produced similar viral titres to those produced by BHK-21 for both strains used. In addition, the yield of rabies glycoprotein was assessed by ELISA. In general, BHK-21 and C6 cells infected either by PV or with the wild rabies strain produced similar amounts of rabies glycoprotein. At 96 hpi, however, when the glycoprotein production peaked, BHK-21 infected with the wild strain produced significantly higher amounts of glycoprotein than C6. Subsequently, the optimal conditions for isolation of wild rabies virus strains from C6 cells were established and these proved to be as sensitive as NA cells in detecting 10 wild rabies samples. Due to the high sensitivity exhibited, C6 rat glioma cells present a new and useful system for rabies virus investigation. |
| Databáze: | OpenAIRE |
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