A Simple Technic for Demonstrating Plaque Formation with Virus of Vaccinia

Autor:	Wilbur Fiske Noyes
Rok vydání:	1953
Předmět:	animal structures viruses Vaccinia virus Embryo Biology Chick embryos Virology General Biochemistry Genetics and Molecular Biology Virus chemistry.chemical_compound Specific antibody Tissue culture chemistry Viruses embryonic structures Viral Activity Vaccinia Humans Embryo tissue
Zdroj:	Experimental Biology and Medicine. 83:426-429
ISSN:	1535-3699 1535-3702
DOI:	10.3181/00379727-83-20378
Popis:	Discussion and SummaryA simple technic is described whereby the production of plaques in chick embryo tissue cultures has been consistently obtained with vaccinia virus. The plaques are probably due to viral activity. This conclusion is supported by the fact that 1) the plaques are absent when the virus is absent and their number increases with the concentration of the virus, 2) the number of plaques produced intissue culture correlates with the number of pox produced on the CAM of the developing chick embryo and 3) specific antibody reduces both the number of plaques formed in tissue culture and the number of pox formed on the CAM by 95%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66b1a25a7a8f486f1c8e6bbc2df330fc https://doi.org/10.3181/00379727-83-20378 Zobrazit plný text záznamu