Antibacterial mechanism and transcriptome analysis of ultra-small gold nanoclusters as an alternative of harmful antibiotics against Gram-negative bacteria
| Autor: | Yangheng Zhang, Peng Wang, Cheng Jiang, Matthew Jay Malkmes, Ling Jiang, Yuxian Wang, Liying Zhu, Hongman Zhang, He Huang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Environmental Engineering
Gram-negative bacteria DNA damage Health Toxicology and Mutagenesis 0211 other engineering and technologies 02 engineering and technology Microbial Sensitivity Tests 010501 environmental sciences medicine.disease_cause 01 natural sciences Transcriptome Anti-Infective Agents Gram-Negative Bacteria medicine Environmental Chemistry Waste Management and Disposal 0105 earth and related environmental sciences 021110 strategic defence & security studies biology Chemistry Gene Expression Profiling DNA replication RNA biology.organism_classification Pollution Anti-Bacterial Agents Citric acid cycle Biochemistry Gold Oxidative stress Bacteria |
| Zdroj: | Journal of hazardous materials. 416 |
| ISSN: | 1873-3336 |
| Popis: | In this work, a well-known Au25 NCs with high purity was prepared by simple one-pot reducing method. The as-synthesized Au25 NCs exhibited excellent antibacterial efficiency toward Gram-negative bacteria in a dose- and time-dependent manner, which could be used as nanoantibiotics to replace harmful antibiotics. The antibacterial assays showed that almost 100% bacteria were killed at lower concentration (100–150 μM) within a short time (30–60 min), providing a rapid and effective killing outcome for Gram-negative bacteria. After that, antibacterial mechanism was mainly investigated at cellular level via destruction of membrane integrity, disruption of antioxidant defense system, metabolic inactivation, DNA damage, as well as at molecular level via transcriptome analysis (RNA sequencing) for the first time. RNA sequencing results showed that differentially expressed genes (DEGs) related to biosynthesis of cell wall and membrane, glycolysis and TCA cycle, oxidative phosphorylation and DNA replication and repair were significantly affected. It was concluded that synergetic effect of membrane damage, oxidative stress, DNA damage and energy metabolism eventually led to the Gram-negative bacteria growth inhibition and death. |
| Databáze: | OpenAIRE |
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