Generation of mice with a novel conditional null allele of the Sox9 gene
| Autor: | Petra Kraus, V. Sivakamasundari, Thomas Lufkin, Hsiao Yun Chan, Sook Peng Yap, Xing Xing |
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| Rok vydání: | 2011 |
| Předmět: |
endocrine system
animal structures Gene Dosage Cre recombinase Bioengineering Biology Applied Microbiology and Biotechnology Gene dosage Gene Knockout Techniques Mice Exon stomatognathic system Forelimb medicine Animals Cloning Molecular Allele Collagen Type II Alleles Mice Knockout Genetics Integrases Models Genetic Histocytochemistry Musculoskeletal Development Wild type Gene Expression Regulation Developmental Gene targeting SOX9 Transcription Factor General Medicine Embryo Mammalian musculoskeletal system medicine.disease Molecular biology Null allele Spine Campomelic dysplasia Gene Targeting embryonic structures Biotechnology |
| Zdroj: | Biotechnology Letters. 33:1551-1558 |
| ISSN: | 1573-6776 0141-5492 |
| Popis: | Sox9 is expressed in multiple tissues during mouse development and adulthood. Mutations in the Sox9 gene or changes in expression levels can be attributed to many congenital diseases. Heterozygous loss-of-function mutations in the human SOX9 gene cause Campomelic dysplasia, a semi-lethal skeletal malformation syndrome. Disruption of Sox9 by conventional gene targeting leads to perinatal lethality in heterozygous mice, hence hampering the feasibility to obtain the homozygous Sox9 null mice for in vivo functional studies. In this study, we generated a conditional allele of Sox9 (Sox9 tm4.Tlu ) by flanking exon 1 with loxP sites. Homozygous mice for the Sox9 tm4.Tlu allele (Sox9 flox/flox ) are viable, fertile and indistinguishable from wildtype (WT) mice, indicating that the Sox9 tm4.Tlu allele is a fully functional Sox9 allele. Furthermore, we demonstrated that Cre-mediated recombination using a Col2a1-Cre line resulted in specific ablation of Sox9 activity in cartilage tissues. |
| Databáze: | OpenAIRE |
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