Improved Method for Detection of Vibrio parahaemolyticus in Seafood
| Autor: | Hirotaka Konuma, Junko Hasegawa, Tokuhiro Nishina, Hiroshi Nakagawa, Susumu Kumagai, Yukiko Hara-Kudo |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
food.ingredient
Food Contamination Public Health Microbiology Biology Applied Microbiology and Biotechnology Microbiology Agar plate chemistry.chemical_compound food Vibrionaceae Thiosulfate-citrate-bile salts-sucrose agar Animals Humans Agar Food science Bacteriological Techniques Growth medium Ecology Chromogenic Vibrio parahaemolyticus Fishes food and beverages equipment and supplies Chromogenic Compounds biology.organism_classification Culture Media Seafood chemistry Mollusca Vibrio Infections Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 67:5819-5823 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.67.12.5819-5823.2001 |
| Popis: | We have developed a new, effective procedure for detecting Vibrio parahaemolyticus in seafoods using enrichment and plating onto a chromogenic agar medium. Samples were cultured in salt Trypticase soy broth, which is a nonselective medium, and then a portion of the culture was cultured with salt polymyxin broth, which is a selective medium for V. parahaemolyticus . This two-step enrichment was more effective than the one-step enrichment in salt polymyxin broth alone. The enrichment cultures were then plated onto a new chromogenic agar containing substrates for beta-galactosidase. The V. parahaemolyticus colonies developed a purple color on this growth medium that distinguished them from other related bacterial strains. V. parahaemolyticus was isolated more frequently from naturally contaminated seafood samples using the chromogenic agar than thiosulfate citrate bile salts sucrose agar medium, which is currently used for the isolation of V. parahaemolyticus . Our findings suggest that this new enrichment and isolation scheme is more sensitive and accurate for identifying V. parahaemolyticus in seafood samples than previously used methods. |
| Databáze: | OpenAIRE |
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