Ultrasensitive photoelectrochemical immunoassay for prostate-specific antigen based on silver nanoparticle-triggered ion-exchange reaction with ZnO/CdS nanorods
| Autor: | Mei-Jin Li, Ling Zhu, Dianping Tang, Zipeng Yin, Zijian Lv |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Silver Metal Nanoparticles 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences Biochemistry Silver nanoparticle Analytical Chemistry Limit of Detection Electrochemistry medicine Environmental Chemistry Humans Spectroscopy Photocurrent Immunoassay Nanotubes medicine.diagnostic_test Ion exchange Chemistry Electrochemical Techniques Prostate-Specific Antigen 021001 nanoscience & nanotechnology 0104 chemical sciences Prostate-specific antigen Linear range Electrode Nanorod Zinc Oxide 0210 nano-technology Nuclear chemistry |
| Zdroj: | The Analyst. 146(14) |
| ISSN: | 1364-5528 |
| Popis: | Prostate-specific antigen (PSA), a glycoprotein that is most likely to cause prostate cancer, has attracted widespread attention in recent years due to its increasing threat to people's lives and health. Herein, we developed a new signal-amplified photoelectrochemical (PEC) immunosensing method for quantitative monitoring of the target PSA based on the ion-exchange reaction for the in situ formation of ZnO/CdS/Ag2S nanohybrids triggered by the as-released silver ions (Ag+) from silver nanolabels. Initially, the introduction of a target PSA caused the formation of a sandwich immunocomplex in an anti-PSA capture antibody (cAb)-coated microplate with the help of a silver nanoparticle-labeled detection antibody (AgNPs-dAb). Thereafter, the introduced AgNPs were dissolved with acid to release numerous silver ions. In this regard, an ion-exchange reaction occurred between the silver ions and ZnO/CdS nanorods on the photosensitive electrode, thus producing ZnO/CdS/Ag2S nanohybrids to generate a relatively strong photocurrent. Under optimal conditions, the ion-exchange reaction-based PEC immunoassay exhibited a good linear range of 0.05–50 ng mL−1 and allowed the detection of the target PSA at a concentration as low as 0.018 ng mL−1. In addition, the PEC immunoassay displayed satisfactory repeatability, high specificity, and acceptable method accuracy. Importantly, the ion-exchange reaction-based PEC immunoassay provides a new perspective for the detection of other disease-related biomarkers by controlling the corresponding antibodies. |
| Databáze: | OpenAIRE |
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