HSF1 and HSF3 cooperatively regulate the heat shock response in lizards
Autor: | Fangxu Wu, Hiroshi D. Akashi, Takashi Makino, Yuki Matsuura, Eiichi Takaki, Arpit Katiyar, Masakado Kawata, Akira Nakai, Mitsuaki Fujimoto, Namiko Oshibe, Ryosuke Takii |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Adenoviruses Hot Temperature lcsh:Medicine Pathology and Laboratory Medicine Biochemistry Heat Shock Response Poultry 0302 clinical medicine Heat Shock Transcription Factors Medicine and Health Sciences Gamefowl lcsh:Science HSF1 Heat-Shock Proteins Phylogeny Cellular Stress Responses Data Management Gene knockdown Multidisciplinary Ecology Vertebrate Lizards Phylogenetic Analysis Complementary DNA Squamates Cell biology Nucleic acids Phylogenetics DNA-Binding Proteins Cell Processes Medical Microbiology Shock (circulatory) Viral Pathogens Vertebrates Viruses Frogs medicine.symptom Pathogens Anura Research Article Computer and Information Sciences DNA Complementary Forms of DNA Biology Microbiology Amphibians Birds Avian Proteins Evolution Molecular 03 medical and health sciences Heat shock protein biology.animal medicine Genetics Animals Evolutionary Systematics Heat shock Microbial Pathogens Taxonomy Evolutionary Biology lcsh:R Organisms Biology and Life Sciences Reptiles Cell Biology DNA Hsp70 Heat shock factor 030104 developmental biology Fowl Amniotes Trans-Activators lcsh:Q DNA viruses Chickens 030217 neurology & neurosurgery Heat-Shock Response Transcription Factors |
Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 7, p e0180776 (2017) |
ISSN: | 1932-6203 |
Popis: | Cells cope with temperature elevations, which cause protein misfolding, by expressing heat shock proteins (HSPs). This adaptive response is called the heat shock response (HSR), and it is regulated mainly by heat shock transcription factor (HSF). Among the four HSF family members in vertebrates, HSF1 is a master regulator of HSP expression during proteotoxic stress including heat shock in mammals, whereas HSF3 is required for the HSR in birds. To examine whether only one of the HSF family members possesses the potential to induce the HSR in vertebrate animals, we isolated cDNA clones encoding lizard and frog HSF genes. The reconstructed phylogenetic tree of vertebrate HSFs demonstrated that HSF3 in one species is unrelated with that in other species. We found that the DNA-binding activity of both HSF1 and HSF3 in lizard and frog cells was induced in response to heat shock. Unexpectedly, overexpression of lizard and frog HSF3 as well as HSF1 induced HSP70 expression in mouse cells during heat shock, indicating that the two factors have the potential to induce the HSR. Furthermore, knockdown of either HSF3 or HSF1 markedly reduced HSP70 induction in lizard cells and resistance to heat shock. These results demonstrated that HSF1 and HSF3 cooperatively regulate the HSR at least in lizards, and suggest complex mechanisms of the HSR in lizards as well as frogs. |
Databáze: | OpenAIRE |
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