HSF1 and HSF3 cooperatively regulate the heat shock response in lizards

Autor: Fangxu Wu, Hiroshi D. Akashi, Takashi Makino, Yuki Matsuura, Eiichi Takaki, Arpit Katiyar, Masakado Kawata, Akira Nakai, Mitsuaki Fujimoto, Namiko Oshibe, Ryosuke Takii
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Adenoviruses
Hot Temperature
lcsh:Medicine
Pathology and Laboratory Medicine
Biochemistry
Heat Shock Response
Poultry
0302 clinical medicine
Heat Shock Transcription Factors
Medicine and Health Sciences
Gamefowl
lcsh:Science
HSF1
Heat-Shock Proteins
Phylogeny
Cellular Stress Responses
Data Management
Gene knockdown
Multidisciplinary
Ecology
Vertebrate
Lizards
Phylogenetic Analysis
Complementary DNA
Squamates
Cell biology
Nucleic acids
Phylogenetics
DNA-Binding Proteins
Cell Processes
Medical Microbiology
Shock (circulatory)
Viral Pathogens
Vertebrates
Viruses
Frogs
medicine.symptom
Pathogens
Anura
Research Article
Computer and Information Sciences
DNA
Complementary

Forms of DNA
Biology
Microbiology
Amphibians
Birds
Avian Proteins
Evolution
Molecular

03 medical and health sciences
Heat shock protein
biology.animal
medicine
Genetics
Animals
Evolutionary Systematics
Heat shock
Microbial Pathogens
Taxonomy
Evolutionary Biology
lcsh:R
Organisms
Biology and Life Sciences
Reptiles
Cell Biology
DNA
Hsp70
Heat shock factor
030104 developmental biology
Fowl
Amniotes
Trans-Activators
lcsh:Q
DNA viruses
Chickens
030217 neurology & neurosurgery
Heat-Shock Response
Transcription Factors
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 7, p e0180776 (2017)
ISSN: 1932-6203
Popis: Cells cope with temperature elevations, which cause protein misfolding, by expressing heat shock proteins (HSPs). This adaptive response is called the heat shock response (HSR), and it is regulated mainly by heat shock transcription factor (HSF). Among the four HSF family members in vertebrates, HSF1 is a master regulator of HSP expression during proteotoxic stress including heat shock in mammals, whereas HSF3 is required for the HSR in birds. To examine whether only one of the HSF family members possesses the potential to induce the HSR in vertebrate animals, we isolated cDNA clones encoding lizard and frog HSF genes. The reconstructed phylogenetic tree of vertebrate HSFs demonstrated that HSF3 in one species is unrelated with that in other species. We found that the DNA-binding activity of both HSF1 and HSF3 in lizard and frog cells was induced in response to heat shock. Unexpectedly, overexpression of lizard and frog HSF3 as well as HSF1 induced HSP70 expression in mouse cells during heat shock, indicating that the two factors have the potential to induce the HSR. Furthermore, knockdown of either HSF3 or HSF1 markedly reduced HSP70 induction in lizard cells and resistance to heat shock. These results demonstrated that HSF1 and HSF3 cooperatively regulate the HSR at least in lizards, and suggest complex mechanisms of the HSR in lizards as well as frogs.
Databáze: OpenAIRE