A bioassay to evaluate the activity of chemical stimuli from grape berries on the oviposition of Lobesia botrana (Lepidoptera: Tortricidae)
| Autor: | Denis Thiéry, N. Maher |
|---|---|
| Přispěvatelé: | Unité Mixte de Recherche en Santé Végétale (INRA/ENITA) (UMRSV), Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut des Sciences de la Vigne et du Vin (ISVV) |
| Rok vydání: | 2004 |
| Předmět: |
0106 biological sciences
Tortricidae Oviposition [SDV.BID]Life Sciences [q-bio]/Biodiversity Berry Moths Lobesia botrana 010603 evolutionary biology 01 natural sciences Lepidoptera genitalia Chemical stimuli Botany Animals Bioassay Vitis ComputingMilieux_MISCELLANEOUS Wax Dose-Response Relationship Drug biology Plant Extracts LEPIDOPTERE General Medicine biology.organism_classification INSECTE 010602 entomology Horticulture Insect Science visual_art visual_art.visual_art_medium Biological Assay Female PEST analysis Agronomy and Crop Science |
| Zdroj: | Bulletin of Entomological Research Bulletin of Entomological Research, Cambridge University Press (CUP), 2004, 94, pp.27-33. ⟨10.1079/BER2003276⟩ |
| ISSN: | 1475-2670 0007-4853 |
| DOI: | 10.1079/ber2003276 |
| Popis: | A two-choice bioassay was developed to evaluate the role of host-plant berry compounds on the oviposition site acceptance of the generalist moth Lobesia botrana (Denis & Shiffermüller). A key feature was the lining of the bioassay arena with felt which focused oviposition on the test substrates. Initial experiments comparing substrates with different physical features indicated that smooth textures and spherical shapes with interstices favour oviposition. Artificial oviposition substrates were thus constructed with glass spheres in order to test the behavioural activity of grapevine berry extracts. Only polar extracts obtained by soaking berries in methanol or water stimulated oviposition (more eggs were laid on the extract-treated substrate than on the control substrate), whereas more apolar ones obtained with chloroform or hexane had no significant effect. The prior removal of epicuticular waxes from grape berries before extraction did not enhance the stimulatory activity of the methanol extract. The oviposition response to this extract was dose-dependent. It is concluded that polar compounds present on grape berries act as oviposition stimulants for L. botrana. |
| Databáze: | OpenAIRE |
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