cycMs3, a novel B-type alfalfa cyclin gene, is induced in the G0-to-G1 transition of the cell cycle

Autor: László Bögre, Heribert Hirt, Marlis Dahl, Manfred Pirck, Erwin Heberle-Bors, Dang Thi Cam Ha, Ines Swoboda, Gustav Ammerer, Irute Meskiene
Jazyk: angličtina
Rok vydání: 1995
Předmět:
DNA Replication
Cyclin D
Molecular Sequence Data
Cyclin A
Cyclin B
Saccharomyces cerevisiae
Plant Science
Resting Phase
Cell Cycle
Pheromones
Cyclin Gene
Cyclin-dependent kinase
Messenger/genetics/metabolism Saccharomyces cerevisiae/cytology/genetics Sequence Homology
Cyclins
Amino Acid Sequence
RNA
Messenger
Plant Proteins
Amino Acid Sequence Cell Division/genetics Cyclins/*genetics DNA Replication/genetics G0 Phase/*genetics G1 Phase/*genetics Medicago sativa/cytology/*genetics Molecular Sequence Data Pheromones/physiology Plant Leaves/cytology/metabolism Plant Proteins/*genetics RNA
Sequence Homology
Amino Acid
biology
G1 Phase
G1/S transition
Cell Biology
Faculty of Science\Biological Science
Cell cycle
Molecular biology
Cell biology
Plant Leaves
Amino Acid
biology.protein
Cell Division
Cyclin A2
Research Article
Medicago sativa
Zdroj: Plant Cell, 7 (6)
Popis: Cyclins are key regulators of the cell cycle in all eukaryotes. We have previously isolated two B-type cyclin genes, cycMs1 and cycMs2, from alfalfa that are primarily expressed during the G2-to-M phase transition and are most likely mitotic cyclin genes. Here, we report the isolation of a novel alfalfa cyclin gene, termed cycMs3 (for cyclin Medicago sativa), by selecting for mating type alpha-pheromone-induced cell cycle arrest suppression in yeast. The central region of the predicted amino acid sequence of the cycMs3 gene is most similar to the cyclin box of yeast B-type and mammalian A- and B-type cyclins. In situ hybridization showed that cycMs3 mRNA can be detected only in proliferating cells and not in differentiated alfalfa cells. When differentiated G0-arrested cells were induced to reenter the cell cycle in the G1 phase and resume cell division by treatment with plant hormones, cycMs3 transcript levels increased long before the onset of DNA synthesis. In contrast, histone H3-1 mRNA and cycMs2 transcripts were not observed before DNA replication and mitosis, respectively. In addition, cycMs3 mRNA was found in all stages of the cell cycle in synchronously dividing cells, whereas the cycMs2 and histone H3-1 genes showed a G2-to-M phase- or S phase-specific transcription pattern, respectively. These data suggest that the role of cyclin CycMs3 differs from that of CycMs1 and CycMs2. We propose that CycMs3 helps control reentry of quiescent G0-arrested cells into the G1 phase of the cell cycle.
Databáze: OpenAIRE
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