Derivation of hybrid ES cell lines from two different strains of mice
| Autor: | Chelsea Ray, Fong T. Bell, Xiajun Li, Ho-Tak Lau, Lizhi Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Heterozygote Genotype Biology Real-Time Polymerase Chain Reaction Article Cell Line Mice 03 medical and health sciences Animals Allele lcsh:QH301-705.5 Embryoid Bodies Embryonic Stem Cells Medicine(all) Genetics Embryo Cell Biology General Medicine DNA Methylation Embryonic stem cell Blastocyst 030104 developmental biology Real-time polymerase chain reaction lcsh:Biology (General) Microscopy Fluorescence Mice Inbred DBA Cell culture DNA methylation Genomic imprinting Transcription Factors Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 16, Iss 2, Pp 252-255 (2016) |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2016.01.017 |
| Popis: | Parental origin-dependent expression of the imprinted genes is essential for mammalian development. Zfp57 maintains genomic imprinting in mouse embryos and ES cells. To examine the allelic expression patterns of the imprinted genes in ES cells, we obtained multiple hybrid ES clones that were directly derived from the blastocysts generated from the cross between mice on two different genetic backgrounds. The blastocyst-derived ES clones displayed largely intact DNA methylation imprint at the tested imprinted regions. These hybrid ES clones will be useful for future studies to examine the allelic expression of the imprinted genes in ES cells and their differentiated progeny. |
| Databáze: | OpenAIRE |
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