Investigation of In vitro PDT Activities and In vivo Biopotential of Zinc Phthalocyanines Using 131 I Radioisotope
Autor: | Fatma Yurt Lambrecht, Cagla Kayabasi, Suleyman Gokhan Colak, Cumhur Gündüz, Kasim Ocakoglu, Hale Melis Soylu, Ozge Er, Mine Ince |
---|---|
Rok vydání: | 2015 |
Předmět: |
endocrine system
Biodistribution Indoles Stereochemistry medicine.medical_treatment chemistry.chemical_element Photodynamic therapy 02 engineering and technology Zinc Isoindoles Biochemistry Iodine Radioisotopes HeLa 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Cell Line Tumor Drug Discovery Organometallic Compounds medicine Animals Humans Tissue Distribution Rats Wistar Pharmacology Photosensitizing Agents Singlet Oxygen biology Singlet oxygen Organic Chemistry Temperature 021001 nanoscience & nanotechnology biology.organism_classification In vitro Rats Photochemotherapy chemistry Zinc Compounds 030220 oncology & carcinogenesis Molecular Medicine Female Chromatography Thin Layer 0210 nano-technology Phototoxicity HeLa Cells Nuclear chemistry |
Zdroj: | Chemical Biology & Drug Design. 87:224-232 |
ISSN: | 1747-0285 1747-0277 |
Popis: | Novel octylthio-containing asymmetrically substituted Zn(II) phthalocyanine (Zn(II)Pc1) and a symmetric derivative (Zn(II)Pc2) have been prepared to investigate the biological potential and ability to photosensitize singlet oxygen for photodynamic therapy applications. In this study, the singlet oxygen generation potential and in vitro photodynamic activities of these compounds have been tested. Both ZnPcs reveal to be very efficient singlet oxygen generators and promising PSs for PDT applications. In vitro PDT activities of the compounds were evaluated in EMT-6 murine mammary carcinoma and HeLa human cervix carcinoma cell lines. Moreover, Zn(II)Pc1 displayed the phototoxic effects in the mammary cancer cell line (6.25 μm concentration at 30 J/cm(2) light dose and 12.5 μm concentration at 20 J/cm(2) light dose), while Zn(II)Pc2 did not show any phototoxic effects both in two cell lines. Zn(II)Pcs were radiolabeled with (131) I in high yields. Biodistribution studies revealed that the radiolabeled Zn(II)Pc1 showed significant uptake in l. intestine, pancreas, brain, and ovary, while Zn(II)Pc2 has significant uptake in ovary and pancreas in normal rats. Hence, these Pcs derivatives could be promising candidate for tumor nuclear imaging. |
Databáze: | OpenAIRE |
Externí odkaz: |