Distribution of HLA DRB1 and DQB1 alleles and DRB1-DQB1 haplotypes among Bahraini women with polycystic ovary syndrome
| Autor: | Zainab H. Malalla, Jawaher A. Al-Jalahma, Safa A. Al-Madhi, Amira Messadi, Wassim Y. Almawi |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
musculoskeletal diseases 0301 basic medicine Genotype endocrine system diseases Immunology Physiology Genome-wide association study Biology Polymerase Chain Reaction Young Adult 03 medical and health sciences 0302 clinical medicine Gene Frequency immune system diseases HLA-DQ beta-Chains Humans Immunology and Allergy Genetic Predisposition to Disease skin and connective tissue diseases HLA-DRB1 Genotyping Allele frequency Genetic Association Studies Retrospective Studies Genetics Polymorphism Genetic 030219 obstetrics & reproductive medicine Haplotype nutritional and metabolic diseases Obstetrics and Gynecology Odds ratio Polycystic ovary 030104 developmental biology Haplotypes Reproductive Medicine Case-Control Studies Bahrain Female HLA-DRB1 Chains Polycystic Ovary Syndrome |
| Zdroj: | Journal of Reproductive Immunology. 117:76-80 |
| ISSN: | 0165-0378 |
| DOI: | 10.1016/j.jri.2016.07.006 |
| Popis: | Objective This study investigated the association between HLA-DRB1 and –DQB1 alleles and DRB1-DQB1 haplotypes, and polycystic ovary syndrome (PCOS) in Bahraini women. Design Case-control, retrospective study. Methods Study subjects comprised 80 women with PCOS, and 169 age- and ethnically-matched control women. DRB1 and DQB1 genotyping was done by PCR-SSP. Results Of the 13 DRB1 alleles and 5 DQB1 alleles identified, DRB1*10 (14.3% vs. 4.4%) and DRB1*14 (8.7% vs. 1.1%), along with DQB1*05 (35.0% vs. 23.9%), were the most frequent alleles in cases, while DRB1*11 (15.3% vs. 6.8%) was the frequent allele found in controls. The association of PCOS with DRB1*10 ( Pc DRB1*14 ( Pc DQB1*05 ( Pc =0.040), but not DRB1*11 ( Pc =0.076) persisted after correcting for multiple comparisons. DRB1-DQB1 haplotype analysis identified nine common shared haplotypes in women with PCOS and control women, with a frequency exceeding 1%. Significantly higher frequency of DRB1*10-DQB1*05 (12.4% vs. 3.1%) and DRB1*14-DQB1*03 (5.6% vs. 1.0%), and reduced frequency of DRB1*11-DQB1*03 (4.1% vs. 14.1%) haplotypes were seen in women with PCOS vs. control women, thus assigning PCOS-susceptible and –protective nature to these haplotypes, respectively. This association persisted after controlling for multiple comparisons. Conclusion Our results confirm an association of HLA-DRB1 and –DQB1 alleles and haplotypes with PCOS susceptibility in Bahraini Arabs, further underscoring the immunological/inflammatory nature of this disorder. |
| Databáze: | OpenAIRE |
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