Direct electron transfer of Trametes hirsuta laccase adsorbed at unmodified nanoporous gold electrodes
| Autor: | Sergey Shleev, Urszula Salaj-Kosla, Edmond Magner, Sascha Pöller, Wolfgang Schuhmann |
|---|---|
| Přispěvatelé: | ERC, HEA, INSPIRE |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
trametes hirsuta laccase Biophysics Enzyme electrode nanoporous gold electrodes Electrons 02 engineering and technology Trametes hirsuta 010402 general chemistry Photochemistry 01 natural sciences Redox Electron Transport Electron transfer Electrochemistry medicine Organic chemistry Physical and Theoretical Chemistry Electrodes Trametes biology Nanoporous Chemistry bioelectrochemistry Laccase General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Enzymes Immobilized Electron transport chain 0104 chemical sciences Nanostructures biocathode Bioelectrochemistry Electrode Gold 0210 nano-technology Oxidation-Reduction Porosity |
| Zdroj: | Bioelectrochemistry (Amsterdam, Netherlands). 91 |
| ISSN: | 1878-562X |
| Popis: | peer-reviewed The enzyme Trametes hirsuta laccase undergoes direct electron transfer at unmodified nanoporous gold electrodes, displaying a current density of 28 mu A/cm(2). The response indicates that ThLc was immobilised at the surface of the nanopores in a manner which promoted direct electron transfer, in contrast to the absence of a response at unmodified polycrystalline gold electrodes. The bioelectrocatalytic activity of ThLc modified nanoporous gold electrodes was strongly dependent on the presence of halide ions. Fluoride completely inhibited the enzymatic response, whereas in the presence of 150 mM Cl-, the current was reduced to 50% of the response in the absence of Cl-. The current increased by 40% when the temperature was increased from 20 degrees C to 37 degrees C. The response is limited by enzymatic and/or enzyme electrode kinetics and is 30% of that observed for ThLc co-immobilised with an osmium redox polymer. (C) 2012 Elsevier B.V. All rights reserved. ACCEPTED peer-reviewed |
| Databáze: | OpenAIRE |
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