Association of the Emerging Carbapenemase NDM-1 with a Bleomycin Resistance Protein in Enterobacteriaceae and Acinetobacter baumannii
| Autor: | Patrice Nordmann, Laurent Poirel, Laurent Dortet |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Acinetobacter baumannii
DNA Bacterial Mutation rate Microbial Sensitivity Tests medicine.disease_cause beta-Lactamases Microbiology Bleomycin Open Reading Frames 03 medical and health sciences Bacterial Proteins Enterobacteriaceae Mechanisms of Resistance Drug Resistance Bacterial Operon Escherichia coli medicine Pharmacology (medical) Insertion sequence Promoter Regions Genetic Gene DNA Primers 030304 developmental biology Pharmacology Genetics chemistry.chemical_classification 0303 health sciences biology 030306 microbiology biology.organism_classification Anti-Bacterial Agents Culture Media Amino acid Multiple drug resistance Imipenem Infectious Diseases chemistry Mutation Indicators and Reagents |
| Zdroj: | Antimicrobial Agents and Chemotherapy Antimicrobial Agents and Chemotherapy; Vol 56 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.05583-11 |
| Popis: | The carbapenemase NDM-1 has been identified recently in Enterobacteriaceae and Acinetobacter baumannii as a source of multidrug resistance, including resistance to carbapenems. By analyzing the immediate genetic environment of the bla NDM-1 carbapenemase gene among a series of NDM-1-producing enterobacterial isolates, a novel gene ( ble MBL , for ble gene associated with the metallo-β-lactamase NDM-1) was identified. The ble MBL gene encodes a novel bleomycin resistance protein (BRP), named BRP MBL , that shares weak similarities with known BRPs (less than 60% amino acid identity). The expression of BRP MBL conferred resistance to bleomycin and to bleomycin-like molecules in Enterobacteriaceae and A. baumannii . The bla NDM-1 and ble MBL genes were coexpressed under the control of the same promoter, located upstream of the bla NDM-1 gene and at the extremity of the insertion sequence IS Aba125 . Most of the NDM producers possessed the ble MBL gene. Although BRP MBL did not modify the growth or death rates of Escherichia coli under experimental conditions, it suppressed the mutation rate of hypermutable E. coli and therefore may stabilize the plasmid-borne bla NDM-1 gene. This study suggests that the emerging carbapenemase NDM-1 is selected by bleomycin-like molecules, and that BRP MBL producers (and consequently NDM producers) are better suited to various environments. |
| Databáze: | OpenAIRE |
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