Characterization of Rat NDRG2 (N-Myc Downstream Regulated Gene 2), a Novel Early Mineralocorticoid-specific Induced Gene
| Autor: | Michel Fay, Frederic Jaisser, Marcel Blot-Chabaud, Maria-Christina Zennaro, Brigitte Escoubet, Nicolette Farman, Nathalie Courtois-Coutry, Sheerazed Boulkroun |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Gene isoform medicine.medical_specialty Kidney Cortex Transcription Genetic Colon medicine.drug_class Molecular Sequence Data Biology Polymerase Chain Reaction Biochemistry Cell Line Rats Sprague-Dawley chemistry.chemical_compound Mineralocorticoids Internal medicine medicine Animals Amino Acid Sequence RNA Messenger Aldosterone Molecular Biology In Situ Hybridization DNA Primers Messenger RNA Kidney Base Sequence Renal sodium reabsorption Myocardium Tumor Suppressor Proteins Sodium Proteins Adrenalectomy Cell Biology Rats Cell biology Kinetics medicine.anatomical_structure Endocrinology Gene Expression Regulation chemistry Organ Specificity Suppression subtractive hybridization Cell culture Mineralocorticoid |
| Zdroj: | Journal of Biological Chemistry. 277:31506-31515 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m200272200 |
| Popis: | The early phase of the stimulatory action of aldosterone on sodium reabsorption in tight epithelia involves hormone-regulated genes that remain to be identified. Using a subtractive hybridization technique on isolated renal cortical collecting ducts from rats injected with a physiological dose of aldosterone, we have identified an early response cDNA highly homologous to human and murine NDRG2 (N-Myc downstream regulated gene 2), which consists of four isoforms and belongs to a new family of differentiation-related genes. NDRG2 mRNA was expressed in classical aldosterone target epithelia, and in the kidney, it was specifically located in the collecting duct, the site of aldosterone-regulated sodium absorption. NDRG2 mRNA was increased within 45 min by aldosterone in the kidney and distal colon, whereas it was unaffected in the heart. In the RCCD2 collecting duct cell line, NDRG2 mRNA was enhanced as early as 15 min after aldosterone addition by transcription-dependent effects. NDRG2 was induced by aldosterone concentrations as low as 10(-9) M, and a maximal effect was observed at 10(-8) M. In contrast, the glucocorticoid dexamethasone was ineffective in NDRG2 expression, whereas the glucocorticoid-regulated gene sgk was induced. Taken together, these results indicate that NDRG2 regulation by aldosterone is an early mineralocorticoid-specific effect. Interestingly, NDRG2 is homologous to Drosophila MESK2, a component of the Ras pathway, suggesting that activation of the Ras cascade may play a significant role in mineralocorticoid signaling. |
| Databáze: | OpenAIRE |
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