Acetylcholinesterase: An enzymatic marker of human red blood cell aging
| Autor: | K. K. Gambhir, Yeato G. Prall, Franklin R. Ampy |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Male medicine.medical_specialty Erythrocytes Aché Biology General Biochemistry Genetics and Molecular Biology Specimen Handling Andrology chemistry.chemical_compound Internal medicine medicine Humans General Pharmacology Toxicology and Pharmaceutics Whole blood chemistry.chemical_classification Anticoagulants Reproducibility of Results Erythrocyte Aging General Medicine Acetylcholinesterase language.human_language Red blood cell medicine.anatomical_structure Endocrinology Enzyme chemistry language Female Hemoglobin Percoll Cell aging Biomarkers |
| Zdroj: | Life Sciences. 63:177-184 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(98)00258-6 |
| Popis: | The purpose of this investigation was to determine whether acetylcholinesterase (AChE) can be used as a marker of cell aging in human red blood cells (RBCs). This study used consented subjects; both males and females in an age range of 21-42 years. The blood samples (8-9 mL) were drawn in tubes containing sodium heparin or EDTA as anticoagulants. To avoid contamination with other cells, (lymphocytes, monocytes and reticulocytes), RBCs were purified (PRBC) by Hypaque-Ficoll gradient technique. The PRBCs were subfractionated into young (y) (1.08-1.09), mid (m) (1.09-1.11) and old (o) (1.11-1.12) percoll density (g/mL) fractions using a discontinuous percoll gradient. The mean +/- 1 SD AChE per gram hemoglobin (U/g Hgb) activities in whole blood (WB) purified human red blood cells (PRBCs), young human red blood cells (y-RBCs), mid age human red blood cells (m-RBCs) and old human red blood cells (o-RBCs) were 27.4 +/- 2.98, 26.0 +/- 2.33, 25.5 +/- 1.64, 20.3 +/- 3.84, 14.6 +/- 3.42 in males and 26.3 +/- 4.44, 24.8 /- 4.83, 26.4 +/- 4.59, 24.0 +/- 5.50 and 12.4 +/- 7.09 in females respectively. Although there was variation in the data, the results indicated that old human red blood cells showed significantly (p |
| Databáze: | OpenAIRE |
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