Surfactant-induced alteration of arachidonic acid metabolism of mammalian cells in culture
| Autor: | Betty Kong, Salvatore J. De Salva, Vincent A. De Leo, Leonard C. Harber |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Cell
Stimulation Arachidonic Acids General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Mice Surface-Active Agents Pulmonary surfactant In vivo medicine Animals Humans Cells Cultured chemistry.chemical_classification Mice Inbred C3H Chromatography Arachidonic Acid biology Fatty acid Metabolism Kinetics medicine.anatomical_structure chemistry Biochemistry biology.protein Prostaglandins Keratins Arachidonic acid Cyclooxygenase Epidermis |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0037-9727 |
| Popis: | Primary irritancy in human and animal skin is characterized by an inflammatory reaction mediated, in part, by membrane-derived arachidonate metabolites. One of the mechanisms of this reaction was investigated in cultured mammalian cells using three surfactants: linear alkyl benzene sulfonate (LAS), alkyl ethoxylate sulfate (AEOS), and TWEEN 20. These compounds listed in order in vivo irritancy are LAS greater than AEOS greater than TWEEN 20. Each of these compounds was studied in C3H-10T1/2 cells and human keratinocytes which had been prelabeled with 3H-labeled arachidonic acid (AA). After labeling, media were removed, cells were washed, and fresh media with or without surfactant were added. Cells were then incubated for 2 hr, media were removed and centrifuged, and an aliquot was assayed by liquid scintillation for release of label. In C3H-10T1/2 cells LAS and AEOS in 5-50 microM concentration stimulated 2 to 10 times the release of [3H]AA as compared to controls. In contrast, concentrations of 50-100 microM of TWEEN were required to release [3H]AA. With keratinocytes the same rank order of surfactant concentrations necessary for release was obtained as found with C3H-10T1/2 cells. High-performance liquid chromatography of media extracts of both cell systems revealed surfactant stimulation of the production of cyclooxygenase AA metabolites. These results confirm the induction of release by primary irritants of fatty acid groups from membrane phospholipids. Subsequent metabolism of these fatty acid groups are an integral part of the primary irritant response. Data presented with three known irritants in this in vitro model show a direct correlation with in vivo studies. |
| Databáze: | OpenAIRE |
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