Shigella in Brazilian children with acute diarrhoea: prevalence, antimicrobial resistance and virulence genes
| Autor: | Edilberto Nogueira Mendes, Luciano Amedée Péret-Filho, Mireille Ângela Bernardes Sousa, Francisco José Penna, Paula Prazeres Magalhães, Guilherme Birchal Collares |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Diarrhea Male Shigellosis lcsh:Arctic medicine. Tropical medicine Genotype Virulence Factors lcsh:RC955-962 lcsh:QR1-502 Virulence medicine.disease_cause Polymerase Chain Reaction lcsh:Microbiology Microbiology Feces dysentery Antibiotic resistance Shigella flexneri Disk Diffusion Antimicrobial Tests Trimethoprim Sulfamethoxazole Drug Combination medicine Prevalence Humans Shigella sonnei Shigella Dysentery Bacillary drug resistance biology Sulfamethoxazole Infant Articles medicine.disease biology.organism_classification Trimethoprim Virology Anti-Bacterial Agents diarrhoea virulence Child Preschool Acute Disease Ampicillin Female Brazil medicine.drug |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 108, Iss 1, Pp 30-35 (2013) Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, Volume: 108, Issue: 1, Pages: 30-35, Published: FEB 2013 |
| ISSN: | 1678-8060 |
| Popis: | Diarrhoeal disease is still considered a major cause of morbidity and mortality among children. Among diarrhoeagenic agents, Shigella should be highlighted due to its prevalence and the severity of the associated disease. Here, we assessed Shigella prevalence, drug susceptibility and virulence factors. Faeces from 157 children with diarrhoea who sought treatment at the Children's Hospital Joao Paulo II, a reference children´s hospital in Belo Horizonte, state of Minas Gerais, Brazil, were cultured and drug susceptibility of the Shigella isolates was determined by the disk diffusion technique. Shigella virulence markers were identified by polymerase chain reaction. The bacterium was recovered from 10.8% of the children (88.2% Shigella sonnei). The ipaH, iuc, sen and ial genes were detected in strains isolated from all shigellosis patients; set1A was only detected in Shigella flexneri. Additionally, patients were infected by Shigella strains of different ial, sat, sen and set1A genotypes. Compared to previous studies, we observed a marked shift in the distribution of species from S. flexneri to S. sonnei and high rates of trimethoprim/sulfamethoxazole resistance. |
| Databáze: | OpenAIRE |
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