Downregulation of adiponectin system in granulosa cells and low levels of HMW adiponectin in PCOS

Autor: Massoud Saidijam, Iraj Amiri, Mehdi Mehdizadeh, Nooshin Shabab, Reza Aflatoonian, Mahnaz Ashrafi, Jamshid Karimi, Mahnaz Yavangi, Sara SoleimaniAsl, Tayebe Artimani
Rok vydání: 2015
Předmět:
Adult
0301 basic medicine
endocrine system
medicine.medical_specialty
Fertilization in Vitro
Controlled ovarian hyperstimulation
Body Mass Index
03 medical and health sciences
Follicle-stimulating hormone
Internal medicine
Genetics
medicine
Humans
Progesterone
Genetics (clinical)
Adiponectin receptor 1
Adiponectin receptor 2
Granulosa Cells
Adiponectin
business.industry
luteinizing hormone/choriogonadotropin receptor
Gene Expression Regulation
Developmental
nutritional and metabolic diseases
Obstetrics and Gynecology
General Medicine
Receptors
LH
Polycystic ovary
Follicular Fluid
Reproductive Physiology and Disease
030104 developmental biology
Endocrinology
Reproductive Medicine
Hormone receptor
Female
Follicle Stimulating Hormone
Receptors
Adiponectin
business
hormones
hormone substitutes
and hormone antagonists
Polycystic Ovary Syndrome
Developmental Biology
Zdroj: Journal of Assisted Reproduction and Genetics. 33:101-110
ISSN: 1573-7330
1058-0468
DOI: 10.1007/s10815-015-0620-1
Popis: The purpose of the study was to investigate changes in adiponectin system expression in granulosa cells (GCs) and high molecular weight adiponectin levels in serum and follicular fluid (FF) of 40 women with polycystic ovary syndrome (PCOS) compared to those in 40 women with normal ovary function. Adiponectin (Adipo), adiponectin receptor 1 (AdipoR1), and adiponectin receptor 2 (AdipoR2) messenger RNA (mRNA) expression levels were measured using quantitative real-time polymerase chain reaction (qRT-PCR). High molecular weight (HMW) adiponectin protein concentration was evaluated by ELISA method. Data were analyzed using Student’s t test and one-way ANOVA in SPSS 21 software. At oocyte retrieval, FF was aspirated and GCs were obtained from a pooled collection of FF per each patient. PCR results showed expression of adiponectin, AdipoR1, AdipoR2, follicle-stimulating hormone receptor (FSHR), and luteinizing hormone receptor (LHR) in GCs. After controlling body mass index (BMI) values, qRT-PCR demonstrated a decreased expression of adiponectin system in GCs of PCOS patients compared to those in controls (p = 0.001). There was a strong positive correlation among AdipoR1 and AdipoR2 expression and also among FSH and LH receptor expression. (Both r = 0.8, p = 0.001). There were low levels of high molecular weight adiponectin in the serum of PCOS patients with controlled ovarian hyperstimulation (30.19 ± 4.3 ng/ml) compared to the controls (48.47 ± 5.9 ng/ml) and in the FF of PCOS patients with controlled ovarian hyperstimulation (7.86 ± 1.44 ng/ml) compared to the controls (14.22 ± 2.01 ng/ml; p = 0.02). Lower expression of adiponectin and its receptors in GCs might be an important manifestation in gonadotropin-stimulated PCOS patients which could influence the physiologic adiponectin roles such as interaction with insulin and LH in induction of GC gene expression.
Databáze: OpenAIRE
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