Phosphorylation-Independent Inhibition of Cdc28p by the Tyrosine Kinase Swe1p in the Morphogenesis Checkpoint
| Autor: | John N. McMillan, Rey A. L. Sia, Elaine S.G. Bardes, Daniel J. Lew |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
G2 Phase
Saccharomyces cerevisiae Proteins Cell cycle checkpoint Genes Fungal Cell Cycle Proteins Saccharomyces cerevisiae Biology CHEK1 Phosphorylation Cell Growth and Development Molecular Biology Alleles DNA Primers Base Sequence Cell Cycle Cell Biology Protein-Tyrosine Kinases Cell cycle G2-M DNA damage checkpoint Actin cytoskeleton Molecular biology Cell biology Wee1 Mutation biology.protein CDC28 Protein Kinase S cerevisiae Tyrosine kinase |
| Zdroj: | Molecular and Cellular Biology. 19:5981-5990 |
| ISSN: | 1098-5549 |
| Popis: | The morphogenesis checkpoint in budding yeast delays cell cycle progression in G(2) when the actin cytoskeleton is perturbed, providing time for cells to complete bud formation prior to mitosis. Checkpoint-induced G(2) arrest involves the inhibition of the master cell cycle regulatory cyclin-dependent kinase, Cdc28p, by the Wee1 family kinase Swe1p. Results of experiments using a nonphosphorylatable CDC28(Y19F) allele suggested that the checkpoint stimulated two inhibitory pathways, one that promoted phosphorylation at tyrosine 19 (Y19) and a poorly characterized second pathway that did not require Cdc28p Y19 phosphorylation. We present the results from a genetic screen for checkpoint-defective mutants that led to the repeated isolation of the dominant CDC28(E12K) allele that is resistant to Swe1p-mediated inhibition. Comparison of this allele with the nonphosphorylatable CDC28(Y19F) allele suggested that Swe1p is still able to inhibit CDC28(Y19F) in a phosphorylation-independent manner and that both the Y19 phosphorylation-dependent and -independent checkpoint pathways in fact reflect Swe1p inhibition of Cdc28p. Remarkably, we found that a Swe1p mutant lacking catalytic activity could significantly delay the cell cycle in vivo during a physiological checkpoint response, even when expressed at single copy. The finding that a Wee1 family kinase expressed at physiological levels can inhibit a nonphosphorylatable cyclin-dependent kinase has broad implications for many checkpoint studies using such mutants in other organisms. |
| Databáze: | OpenAIRE |
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