Altered leukotriene B4 levels by HL-60 cells after monocytic/macrophage differentiation
| Autor: | Joan Roselló-Catafau, I. Ramis, Emilio Gelpí, Georgina Hotter, Daniel Closa |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Leukotriene B4
Cellular differentiation Lipoxygenase Immunology Arachidonic Acids Toxicology Dinoprostone Monocytes Cell Line chemistry.chemical_compound Hydroxyeicosatetraenoic Acids medicine Humans Masoprocol Macrophage Pharmacology (medical) Pharmacology CD40 biology Cell growth Macrophages Monocyte Cell Differentiation Cell biology Thromboxane B2 Nordihydroguaiaretic acid medicine.anatomical_structure chemistry Biochemistry Prostaglandin-Endoperoxide Synthases Cell culture biology.protein Tetradecanoylphorbol Acetate Cell Division |
| Zdroj: | Agents and Actions. 40:72-77 |
| ISSN: | 1420-908X 0065-4299 |
| DOI: | 10.1007/bf01976754 |
| Popis: | The differentiation of HL-60 human promyelocytic leukaemia cells into specific monocytic or granulocytic lineage cells depending of the inductor agent is accompanied by selective regulation of several key enzymes involved in the synthesis of eicosanoids. In this communication we have investigated the changes in arachidonic acid metabolic profiles during phorbol 12-myristate 13-acetate (PMA)-induced monocytic differentiation of HL-60 cells. Our results show that HL-60 cells have spontaneous capacity to synthesize large amounts of LTB4, but PMA-differentiated cells lose the ability to release LTB4. Significant differences are found between HL-60 cells and PMA-treated cells in basal conditions and under ionophore stimulation. The addition of LTB4 at the time of PMA differentiation did not have effects on cell proliferation, but nordihydroguaiaretic acid (NDGA), a potent 5-lipoxygenase inhibitor, also inhibited HL-60 cell proliferation and did not have any effect on PMA-differentiated cell proliferation. |
| Databáze: | OpenAIRE |
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