Staining Pattern of the Brush Border and Detection of Cytoplasmic Granules in the Uriniferous Tubules of Female DBA/2Cr Mouse Kidney: Comparison among Various Fixations and Stains
| Autor: | Yoshihiro Hayashi, Masamichi Kurohmaru, Syusaku Suzuki, Hayao Nishinakagawa, Akira Yabuki, Mitsuharu Matsumoto |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Pathology
medicine.medical_specialty Brush border Periodic acid–Schiff stain Biology Cytoplasmic Granules Carnoy's solution Kidney Tubules Proximal Fixatives Mice chemistry.chemical_compound stomatognathic system medicine Animals Toluidine Coloring Agents Fixative Microvilli Staining and Labeling General Veterinary Periodic acid Congo red Staining chemistry Mice Inbred DBA Female |
| Zdroj: | Journal of Veterinary Medical Science. 63:1339-1342 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.63.1339 |
| Popis: | The proximal straight tubules of the female mouse kidney exhibit heavy periodic acid Schiff (PAS) staining in their brush borders and numerous cytoplasmic granules. In the present study, the female DBA/2Cr mouse kidney was examined, using various fixatives (formalin, PFA, PLP, Zamboni's, Bouin, or Carnoy solution) and various staining methods (HE, PAS, alcian blue, periodic acid methenamine-silver (PAM), toluidine blue, azan, or Congo red). Under azan and PAM, the staining pattern of the brush border was similar to that of PAS, and few effects of the fixative were observed. Cytoplasmic granules were clearly detected with PAM as well as PAS. However, these granules were not detected with Carnoy solution. Furthermore, distribution of granules differed between PAS and PAM. |
| Databáze: | OpenAIRE |
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