Crystallin Gene Expression and Lentoid Body Formation in Quail Embryo Neuroretina Cultures Transformed by the oNcogenic Retrovirus Mill Hill 2 or Rous Sarcoma Virus
| Autor: | Alliot F, L Simonneau, B Pessac, Georges Calothy, P Crisanti, A M Lorinet, Y Courtois |
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| Rok vydání: | 1986 |
| Předmět: |
Embryo
Nonmammalian animal structures Genes Viral Transcription Genetic Cellular differentiation Coturnix Avian sarcoma virus Embryonic and Fetal Development Crystallin biology.animal Lens Crystalline Animals RNA Messenger Lentoid Molecular Biology Rous sarcoma virus biology Embryo Oncogenes Cell Biology biology.organism_classification Crystallins Virology Quail eye diseases Cell biology Cell Transformation Neoplastic Retroviridae Avian Sarcoma Viruses Genes embryonic structures sense organs Research Article |
| Zdroj: | Molecular and Cellular Biology. 6:3704-3710 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.6.11.3704-3710.1986 |
| Popis: | The lens-specific proteins alpha and delta crystallins and lentoid bodies, structures that follow a differentiation pathway similar to that of the lens, regularly appear after 4 to 5 weeks in quail embryo neuroretina monolayer cultures. We have investigated the effects of the avian oncogenic retroviruses Mill Hill 2 and Rous sarcoma virus on this process. Quail embryo neuroretina cells transformed by Mill Hill 2 virus were established into permanent cultures that synthesized alpha and delta crystallins and contained stem cells for the production of lentoid bodies. In contrast, transformation with the Rous sarcoma virus mutant tsNY-68 blocked the appearance of mRNA crystallins, but cytoplasmic alpha and delta crystallin mRNA and alpha crystallin appeared 44 h after a shift to the nonpermissive temperature. However, delta crystallins and lentoid bodies were only present after 7 days. The crystallins of transformed quail neuroretina cultures were immunologically indistinguishable from those of quail lenses and of normal quail embryo neuroretina cultures. |
| Databáze: | OpenAIRE |
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