Molecular cloning of a calmodulin-dependent phosphatase from murine testis: identification of a developmentally expressed nonneural isoenzyme
| Autor: | Susumu Higuchi, Randall L. Kincaid, Taro Muramatsu, Polavarapu Rathna Giri |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Gene isoform Molecular Sequence Data Restriction Mapping Phosphatase Gene Expression Biology Mice Complementary DNA Testis Gene expression Phosphoprotein Phosphatases Animals Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence Southern blot Messenger RNA Multidisciplinary Base Sequence Calcineurin Blotting Northern Molecular biology Isoenzymes Blotting Southern Open reading frame Genes Oligodeoxyribonucleotides Calmodulin-Binding Proteins Sequence Alignment Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 89:529-533 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.89.2.529 |
| Popis: | A unique isoform of the catalytic subunit of calmodulin-dependent protein phosphatase (CaM-PrP) was cloned from a murine testis library. The cDNA sequence of 1964 base pairs contained an open reading frame encoding a protein of 513 amino acids (Mr approximately 58,706), the predicted isoelectric point of which (pI 7.1) was much more basic than those of brain isoforms (pI 5.6-5.8). The deduced amino acid sequence was 77-81% identical to two other murine CaM-PrP genes and displayed a distinct Southern blot hybridization pattern, indicating that it was derived from a separate gene (type 3). High amounts of a 2800-nucleotide mRNA transcript were observed in testis, whereas mRNA species were not detectable in brain; thus, it seems likely that this CaM-PrP represents a nonneural isoenzyme. Measurements of CaM-PrP mRNA during testicular development showed a dramatic increase in expression during weeks 4-6, correlating with the later stages of spermatogenesis. These data suggest that this phosphatase isoform may be involved in germ-cell function and are consistent with the report of a flagellum-associated form of CaM-PrP that may regulate sperm motility [Tash, J. S., Krinks, M., Patel, J., Means, R. L., Klee, C. B. & Means, A. R. (1988) J. Cell Biol. 106, 1625-1633]. |
| Databáze: | OpenAIRE |
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