Electrostimulation in an autonomous culture lab-on-chip provides neuroprotection of a retinal explant from a retinitis pigmentosa mouse-model
| Autor: | Carmen Aracil, Francisco J. Diaz-Corrales, Jose M. Quero, Isabel Relimpio, S. S. Bhattacharya, Francisco Perdigones, Lourdes Valdés-Sánchez, Miguel Cabello, Marta Mozo, Berta de la Cerda |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, European Commission, Junta de Andalucía, Fundación Progreso y Salud |
| Rok vydání: | 2019 |
| Předmět: |
02 engineering and technology
010402 general chemistry 01 natural sciences Photoreceptor cell law.invention chemistry.chemical_compound Retinitis-Pigmentosa law Retinitis pigmentosa Materials Chemistry medicine Lab-on-chip Electrical and Electronic Engineering Organotypic-culture Instrumentation Polydimethylsiloxane Metals and Alloys Retinal Multielectrode array Lab-on-a-chip 021001 nanoscience & nanotechnology Condensed Matter Physics medicine.disease Neuroprotection 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Microelectrode medicine.anatomical_structure chemistry Electrostimulation Micro-incubator 0210 nano-technology Biomedical engineering Explant culture |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0925-4005 |
| Popis: | In this paper an autonomous culture system with the capability to electrostimulate organotypic cultures is described, and its utility is demonstrated by the neuroprotection achieved in retinal explants. The system is composed of a lab-on-chip (LOC), an electronic circuit and a data acquisition device. The LOC, in which the culture takes place, includes a microelectrode array (MEA), consisting of a PCB with a group of gold microelectrodes embedded in polydimethylsiloxane (PDMS), a microheater, a thermistor and a microfluidic circuit made of thermoplastic for feeding with culture medium. A plug made of PDMS has been included to facilitate the assembly of the culture LOC, the placement of the mouse retinas inside the MEA and the flow of culture medium. The transparency of the PDMS permits optical applications and a real time monitoring. An electronic circuit allows for a close monitoring of the experiment using a LabVIEW software specifically developed for this setting, including temperature control, heating and electrostimulation. In the experimental conditions, the retinal explants from retinitis pigmentosa mouse-models are dissected the day before neurodegeneration starts, when photoreceptor cell death is expected to progress along the following days, and cultured inside the LOC, using a fluorophore as a live-dead marker. The stimulation is a biphasic square signal of 0.5 Vpp and it is applied for five minutes every other day. Unstimulated RP explants and healthy retinas are used as controls. After seven days, a histological study is performed. We have demonstrated the applicability of this system as an organotypic culture LOC to test the effect of electrostimulation in retinal explants from different mouse models, and found a protective effect on photoreceptor cell death in the conditions tested. This work was supported by the Spanish Ministry of Science and Innovation (project TEC2014-54449-C3-2-R, BIOLOP), by ISCIII (Miguel Servet-I, 2015) cofinanced by the European Regional Development Fund (ERDF) (CP15/00071) and the Andalusian Health Ministry (Fundacion Publica Andaluza Progreso y Salud). |
| Databáze: | OpenAIRE |
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