Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
| Autor: | Koula Sourris, Kiymet Bozaoglu, Freya Bruveris, Penelope McDonald, Edouard G. Stanley, Andrew G. Elefanty, Robyn Mayberry, Katerina Vlahos, Jacqueline V. Schiesser, Paul J. Lockhart |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Mesoderm Induced Pluripotent Stem Cells Cell Culture Techniques Biology Peripheral blood mononuclear cell Cell Line Kruppel-Like Factor 4 Young Adult 03 medical and health sciences 0302 clinical medicine SOX2 medicine Humans Induced pluripotent stem cell lcsh:QH301-705.5 fungi Karyotype Cell Biology General Medicine Middle Aged Molecular biology 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) KLF4 Cell culture embryonic structures Leukocytes Mononuclear Female Reprogramming 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 34, Iss, Pp-(2019) |
| ISSN: | 1873-5061 |
| Popis: | We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms. |
| Databáze: | OpenAIRE |
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