Fluorescein Isothiocyanate (FITC)‐Dextran Extravasation as a Measure of Blood‐Brain Barrier Permeability
| Autor: | Nicole A. Northrop, Bryan K. Yamamoto, Reka Natarajan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Blood–brain barrier Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Fluorescein isothiocyanate General Neuroscience Brain Endothelial Cells Biological Transport Dextrans Extravasation Capillaries Rats Perfusion 030104 developmental biology Dextran medicine.anatomical_structure nervous system chemistry Blood-Brain Barrier Permeability (electromagnetism) Microvessels cardiovascular system Biophysics Blood brain barrier permeability Neuroscience Fluorescein-5-isothiocyanate 030217 neurology & neurosurgery Homeostasis |
| Zdroj: | Current Protocols in Neuroscience |
| ISSN: | 1934-8576 1934-8584 |
| DOI: | 10.1002/cpns.25 |
| Popis: | The blood-brain barrier (BBB) is formed in part by vascular endothelial cells that constitute the capillaries and microvessels of the brain. The function of this barrier is to maintain homeostasis within the brain microenvironment and buffer the brain from changes in the periphery. A dysfunction of the BBB would permit circulating molecules and pathogens typically restricted to the periphery to enter the brain and interfere with normal brain function. As increased permeability of the BBB is associated with several neuropathologies, it is important to have a reliable and sensitive method that determines BBB permeability and the degree of BBB disruption. A detailed protocol is presented for assessing the integrity of the BBB by transcardial perfusion of a 10,000 Da FITC-labeled dextran molecule and its visualization to determine the degree of extravasation from brain microvessels. © 2017 by John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text