DNA Deformation Energy as an Indirect Recognition Mechanism in Protein-DNA Interactions
| Autor: | M. Johnson, Richard H. Lathrop, Donald F. Senear, Kimberly A. Aeling, N.R. Steffen, G.W. Hatfield |
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| Rok vydání: | 2007 |
| Předmět: |
DNA
Bacterial Integration Host Factors Models Molecular Serum Response Factor Work (thermodynamics) Cyclic AMP Receptor Protein Base pair Plasma protein binding Deformation (meteorology) Biology chemistry.chemical_compound DNA Algal Genetics Animals Humans Binding site Binding Sites Endodeoxyribonucleases Base Sequence Applied Mathematics Molecular biophysics DNA DNA Restriction Enzymes DNA Protozoan DNA-Binding Proteins genomic DNA Models Chemical chemistry Biophysics Thermodynamics Algorithms Protein Binding Biotechnology |
| Zdroj: | IEEE/ACM Transactions on Computational Biology and Bioinformatics. 4:117-125 |
| ISSN: | 1545-5963 |
| DOI: | 10.1109/tcbb.2007.1000 |
| Popis: | Proteins that bind to specific locations in genomic DNA control many basic cellular functions. Proteins detect their binding sites using both direct and indirect recognition mechanisms. Deformation energy, which models the energy required to bend DNA from its native shape to its shape when bound to a protein, has been shown to be an indirect recognition mechanism for one particular protein, Integration Host Factor (IHF). This work extends the analysis of deformation to two other DNA-binding proteins, CRP and SRF, and two endonucleases, I-CreI and I-PpoI. Known binding sites for all five proteins showed statistically significant differences in mean deformation energy as compared to random sequences. Binding sites for the three DNA-binding proteins and one of the endonucleases had mean deformation energies lower than random sequences. Binding sites for I-PpoI had mean deformation energy higher than random sequences. Classifiers that were trained using the deformation energy at each base pair step showed good cross-validated accuracy when classifying unseen sequences as binders or nonbinders. These results support DNA deformation energy as an indirect recognition mechanism across a wider range of DNA-binding proteins. Deformation energy may also have a predictive capacity for the underlying catalytic mechanism of DNA-binding enzymes. |
| Databáze: | OpenAIRE |
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