Non-saponifiable fraction of cocoa shell butter: effect on rat and human skin fibroblasts
| Autor: | R. Warocquier-Clerout, H. Chaveron, M. Ouraghi, M. Sigot |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
Aging Chromatography Pharmaceutical Science Human skin Biological activity Fraction (chemistry) Dermatology Biology chemistry.chemical_compound Colloid and Surface Chemistry medicine.anatomical_structure chemistry Unsaponifiable Biochemistry Chemistry (miscellaneous) Drug Discovery Toxicity medicine Dimethylformamide lipids (amino acids peptides and proteins) Fibroblast Carotenoid |
| Zdroj: | International Journal of Cosmetic Science. 14:39-46 |
| ISSN: | 1468-2494 0142-5463 |
| DOI: | 10.1111/j.1467-2494.1992.tb00038.x |
| Popis: | Synopsis Non-saponifiable lipid fraction (ICSB) extracted from cocoa shell butter was solubilized in dimethylformamide (DMF) and analysed for its biological activity on growth of rat and human fibroblasts. Non-saponifiables (10 mug ml(-1)) partially protected cells from toxicity of DMF (1%) and allowed the growth of fibroblasts cultivated in optimal conditions (10% fetal calf serum-FCS, 37 degrees C) or improved the survival of cells maintained in altered conditions (2.5% FCS, 35 degrees C). At higher concentration (ICSB 50 mug ml(-1), DMF 1%), the protective effect was suppressed. ICSB was fractionated by chromatography into four compounds: sterols, terpenic alcohols, tocopherols and hydrocarbons +/- carotenoids. We found that biological activity of ICSB was mostly due to the major fraction containing sterols. |
| Databáze: | OpenAIRE |
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