Protective immunity against herpes simplex virus generated by DNA vaccination compared to natural infection
| Autor: | Petra H. Nass, Karen L. Elkins, Jerry P. Weir |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Cellular immunity viruses Lymphocyte Mice Nude Herpesvirus 1 Human Herpesvirus Vaccines Mice SCID Biology medicine.disease_cause Antibodies Viral Virus DNA vaccination Mice Viral Envelope Proteins T-Lymphocyte Subsets medicine Vaccines DNA Animals Mice Knockout B-Lymphocytes General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health Herpes Simplex Virology Molecular biology Mice Inbred C57BL Infectious Diseases medicine.anatomical_structure Herpes simplex virus Immunization Immunoglobulin G biology.protein Molecular Medicine Antibody CD8 |
| Zdroj: | Vaccine. 19(11-12) |
| ISSN: | 0264-410X |
| Popis: | To evaluate the utility of plasmid DNA vaccination against disease caused by herpes simplex virus (HSV), we compared the strength of protection against lethal challenge following natural virus infection with that following vaccination with a plasmid encoding HSV glycoprotein gD (gD-DNA). We further determined the cellular basis of each type of protection using lymphocyte deficient knockout mice. Establishment of immunity to HSV using live virus immunization required CD8 + T cells and B cells, but not CD4 + or γ/δ + T cells, and was related to specific antibody levels; surprisingly, CD4 knockout mice had large quantities of IgG anti-HSV serum antibodies. Establishment of immunity to HSV using gD-DNA immunization approached the strength of that generated following sublethal infection, but was dependent on α/β + CD4 + T cells, CD8 + T cells, B cells, and even partially on γ/δ + T cells, and not strictly correlated with antibody levels. |
| Databáze: | OpenAIRE |
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