Molecular Characterization of Brazilian Infectious Bursal Disease Virus Isolated from 1997 to 2005
| Autor: | Clarice Weis Arns, N. M. S. Q. Gama, Isabela Cristina Simoni, Maria Beatriz Cardoso de Oliveira, Ricardo Harakava, E. B. Rivas, E. N.C. Tessari, A. M. I. Kanashiro, Maria Judite Bittencourt Fernandes, Maria Gabriela Vogel |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Sequence analysis Molecular Sequence Data Virulence Biology Infectious bursal disease virus Virus Infectious bursal disease Microbiology Food Animals Phylogenetics Molecular genetics medicine Animals Amino Acid Sequence Phylogeny Poultry Diseases Retrospective Studies Viral Structural Proteins General Immunology and Microbiology Phylogenetic tree Building and Construction Birnaviridae Infections medicine.disease Virology Animal Science and Zoology Flock Chickens Brazil |
| Zdroj: | ResearcherID |
| ISSN: | 1933-5334 |
| DOI: | 10.1637/8933.1 |
| Popis: | SUMMARY. This retrospective study concerned 41 infectious bursal disease virus (IBDV) isolates obtained from Brazilian broiler and layers flocks by reverse transcription-polymerase chain reaction. Twenty-five of them were identified as very virulent (vv) by restriction enzyme analysis and by further nucleotide and phylogenetic analysis. All of them had the typical amino acid residues, and all clustered in a phylogenetic tree with the vvIBDV strains. Four amino acid substitutions, at positions D213N, G254D, S317R, and D323E, were common to 3 vv isolates, Br/03/DB, Br/03/CK, and Br/04/CR, and differed from other vv isolates and strains. These isolates came from the same locale, but were collected in different years, indicating that the vvIBDVs circulating on Brazilian farms are undergoing slight but continuous exchanges. |
| Databáze: | OpenAIRE |
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