Developmental origin of preBötzinger complex respiratory neurons
| Autor: | Guang Y Ling, Srinivasan Tupal, Marina Cristina D Picardo, John A. Hayes, Christopher A. Del Negro, Isabel Llona, Joshua G. Corbin, Sarah E. Ross, Paul A. Gray, Tsutomu Hirata, Jaime Eugenín |
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| Rok vydání: | 2010 |
| Předmět: |
endocrine system
Neurogenesis Stimulation Mice Transgenic Biology Serotonergic Article Glutamatergic Mice Organ Culture Techniques Neural Stem Cells Animals Receptors Somatostatin Medulla Homeodomain Proteins Neurons General Neuroscience Respiratory center Gene Expression Regulation Developmental Cell Differentiation Receptors Neurokinin-1 Respiratory Center Neural stem cell nervous system Respiratory Physiological Phenomena DBX1 Somatostatin Neuroscience |
| Zdroj: | JOURNAL OF NEUROSCIENCE Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 1529-2401 |
| Popis: | A subset of preBötzinger Complex (preBötC) neurokinin 1 receptor (NK1R) and somatostatin peptide (SST)-expressing neurons are necessary for breathing in adult rats,in vivo. Their developmental origins and relationship to other preBötC glutamatergic neurons are unknown. Here we show, in mice, that the “core” of preBötC SST+/NK1R+/SST 2a receptor+(SST2aR) neurons, are derived from Dbx1-expressing progenitors. We also show that Dbx1-derived neurons heterogeneously coexpress NK1R and SST2aR within and beyond the borders of preBötC. More striking, we find that nearly all non-catecholaminergic glutamatergic neurons of the ventrolateral medulla (VLM) are also Dbx1 derived. PreBötC SST+neurons are born between E9.5 and E11.5 in the same proportion as non-SST-expressing neurons. Additionally, preBötC Dbx1 neurons are respiratory modulated and show an early inspiratory phase of firing in rhythmically active slice preparations. Loss of Dbx1 eliminates all glutamatergic neurons from the respiratory VLM including preBötC NK1R+/SST+neurons. Dbx1 mutant mice do not express any spontaneous respiratory behaviorsin vivo. Moreover, they do not generate rhythmic inspiratory activity in isolateden blocpreparations even after acidic or serotonergic stimulation. These data indicate that preBötC core neurons represent a subset of a larger, more heterogeneous population of VLM Dbx1-derived neurons. These data indicate that Dbx1-derived neurons are essential for the expression and, we hypothesize, are responsible for the generation of respiratory behavior bothin vitroandin vivo. |
| Databáze: | OpenAIRE |
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