Cutting Edge: Serotonin Is a Chemotactic Factor for Eosinophils and Functions Additively with Eotaxin
| Autor: | Lyudmila Sikora, Francisco M. Lio, Savita P. Rao, Karine Lavrador, Terlika S. Pandit, P. Sriramarao, Stefen A. Boehme |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Chemokine CCL11
Eotaxin Serotonin medicine.medical_specialty medicine.drug_class Chemotactic Factors Eosinophil Receptors CCR3 Immunology CCR3 Cyproheptadine Dose-Response Relationship Immunologic Adjuvants Immunologic Piperidines immune system diseases Internal medicine Serotonin 5-HT2 Receptor Antagonists medicine Humans Immunology and Allergy Leukocyte Rolling Pulmonary Eosinophilia Receptor Chemistry hemic and immune systems Allergens respiratory system Eosinophil Receptor antagonist Eosinophils Fluorobenzenes Chemotaxis Leukocyte Endocrinology medicine.anatomical_structure Chemokines CC Cell Migration Inhibition Receptors Chemokine Serotonin Antagonists medicine.drug |
| Zdroj: | The Journal of Immunology. 173:3599-3603 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.173.6.3599 |
| Popis: | Elevated levels of serotonin (5-hydroxytryptamine, 5-HT) are observed in the serum of asthmatics. Herein, we demonstrate that 5-HT functions independently as an eosinophil chemoattractant that acts additively with eotaxin. 5-HT2A receptor antagonists (including MDL-100907 and cyproheptadine (CYP)) were found to inhibit 5-HT-induced, but not eotaxin-induced migration. Intravital microscopy studies revealed that eosinophils roll in response to 5-HT in venules under conditions of physiological shear stress, which could be blocked by pretreating eosinophils with CYP. OVA-induced pulmonary eosinophilia in wild-type mice was significantly inhibited using CYP alone and maximally in combination with a CCR3 receptor antagonist. Interestingly, OVA-induced pulmonary eosinophilia in eotaxin-knockout (Eot−/−) mice was inhibited by treatment with the 5-HT2A but not CCR3 receptor antagonist. These results suggest that 5-HT is a potent eosinophil-active chemoattractant that can function additively with eotaxin and a dual CCR3/5-HT2A receptor antagonist may be more effective in blocking allergen-induced eosinophil recruitment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|