Comparative analysis of the widespread and conserved PB1-like viruses infectingPseudomonas aeruginosa
| Autor: | Konstantin A. Miroshnikov, Pieter-Jan Ceyssens, Jean-Paul Noben, Guido Volckaert, Wesley Mattheus, Simon Vanderschraeghe, Johan Robben, Rob Lavigne, Andrew M. Kropinski, Vadim V. Mesyanzhinov, N. N. Sykilinda, Victor N. Krylov |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Transcription
Genetic viruses Myoviridae Biology Microbiology Genome Mass Spectrometry Frameshift mutation Bacteriophage Viral Proteins Conserved Sequence Ecology Evolution Behavior and Systematics Genetic diversity Gene Expression Profiling Virion Genetic Variation Sequence Analysis DNA biology.organism_classification Europe Lytic cycle Capsid DNA Viral Pseudomonas aeruginosa Horizontal gene transfer Pseudomonas Phages Protein Processing Post-Translational |
| Zdroj: | Environmental Microbiology. 11:2874-2883 |
| ISSN: | 1462-2920 1462-2912 |
| DOI: | 10.1111/j.1462-2920.2009.02030.x |
| Popis: | Summary We examined the genetic diversity of lytic Pseudomonas aeruginosa bacteriophage PB1 and four closely related phages (LBL3, LMA2, 14-1 and SN) isolated throughout Europe. They all encapsulate linear, non-permuted genomes between 64 427 and 66 530 bp within a solid, acid-resistant isometric capsid (diameter: 74 nm) and carry non-flexible, contractile tails of approximately 140 nm. The genomes are organized into at least seven transcriptional blocks, alternating on both strands, and encode between 88 (LBL3) and 95 (LMA2) proteins. Their virion particles are composed of at least 22 different proteins, which were identified using mass spectrometry. Post-translational modifications were suggested for two proteins, and a frameshift hotspot was identified within ORF42, encoding a structural protein. Despite large temporal and spatial separations between phage isolations, very high sequence similarity and limited horizontal gene transfer were found between the individual viruses. These PB1-like viruses constitute a new genus of environmentally very widespread phages within the Myoviridae. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text