Vaccine efficacy of transcutaneous immunization with amyloid β using a dissolving microneedle array in a mouse model of Alzheimer's disease
| Autor: | Naoki Okada, Hideaki Okamoto, Yasuaki Kawai, Sachiko Hirobe, Shinsaku Nakagawa, Fumio Kamiyama, Kazuhiko Matsuo, Ying-Shu Quan |
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| Rok vydání: | 2014 |
| Předmět: |
Time Factors
Immunology Mice Transgenic Administration Cutaneous Amyloid beta-Protein Precursor Mice Immune system Antigen Alzheimer Disease Escape Reaction Presenilin-1 Reaction Time medicine Animals Humans Immunology and Allergy Maze Learning Adverse effect Amyloid beta-Peptides business.industry Brain Immunotherapy Active Meningoencephalitis medicine.disease Vaccine efficacy Peptide Fragments Vaccine therapy Vaccination Disease Models Animal Neurology Immunization Immunoglobulin G Mutation Exploratory Behavior Neurology (clinical) business |
| Zdroj: | Journal of Neuroimmunology. 266:1-11 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/j.jneuroim.2013.11.002 |
| Popis: | Vaccine therapy for Alzheimer's disease (AD) based on the amyloid cascade hypothesis has recently attracted attention for treating AD. Injectable immunization using amyloid β peptide (Aβ) comprising 1-42 amino-acid residues (Aβ1-42) as antigens showed therapeutic efficacy in mice; however, the clinical trial of this injected Aβ1-42 vaccine was stopped due to the incidence of meningoencephalitis caused by excess activation of Th1 cells infiltrating the brain as a serious adverse reaction. Because recent studies have suggested that transcutaneous immunization (TCI) is likely to elicit Th2-dominant immune responses, TCI is expected to be effective in treating AD without inducing adverse reactions. Previously reported TCI procedures employed complicated and impractical vaccination procedures; therefore, a simple, easy-to-use, and novel TCI approach needs to be established. In this study, we investigated the vaccine efficacy of an Aβ1-42-containing TCI using our novel dissolving microneedle array (MicroHyala; MH) against AD. MH-based TCI induced anti-Aβ1-42 immune responses by simple and low-invasive application of Aβ1-42-containing MH to the skin. Unfortunately, this TCI system resulted in little significant improvement in cognitive function and Th2-dominant immune responses, suggesting the need for further modification. |
| Databáze: | OpenAIRE |
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