Gene expression profiles during early differentiation of mouse embryonic stem cells
| Autor: | Carl S. Daly, Martin J. Evans, Anna L Hurley, Michael A. Wride, Susan MacLean Hunter, Fiona C. Mansergh |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
KOSR
Gene Expression Profiling Cellular differentiation Cell Differentiation Embryoid body Biology Embryonic stem cell Molecular biology Cell aggregation Mice Inbred C57BL Mice medicine.anatomical_structure lcsh:Biology (General) embryonic structures medicine Animals Stem cell Endoderm Developmental biology lcsh:QH301-705.5 Embryonic Stem Cells Oligonucleotide Array Sequence Analysis Research Article Developmental Biology |
| Zdroj: | BMC Developmental Biology, Vol 9, Iss 1, p 5 (2009) BMC Developmental Biology |
| Popis: | Background Understanding the mechanisms controlling stem cell differentiation is the key to future advances in tissue and organ regeneration. Embryonic stem (ES) cell differentiation can be triggered by embryoid body (EB) formation, which involves ES cell aggregation in suspension. EB growth in the absence of leukaemia inhibitory factor (LIF) leads EBs to mimic early embryonic development, giving rise to markers representative of endoderm, mesoderm and ectoderm. Here, we have used microarrays to investigate differences in gene expression between 3 undifferentiated ES cell lines, and also between undifferentiated ES cells and Day 1–4 EBs Results An initial array study identified 4 gene expression changes between 3 undifferentiated ES cell lines. Tissue culture conditions for ES differentiation were then optimized to give the maximum range of gene expression and growth. -Undifferentiated ES cells and EBs cultured with and without LIF at each day for 4 days were subjected to microarray analysis. -Differential expression of 23 genes was identified. 13 of these were also differentially regulated in a separate array comparison between undifferentiated ES cells and compartments of very early embryos. A high degree of inter-replicate variability was noted when confirming array results. Using a panel of marker genes, RNA amplification and RT-PCR, we examined expression pattern variation between individual -D4-Lif EBs. We found that individual EBs selected from the same dish were highly variable in gene expression profile. Conclusion ES cell lines derived from different mouse strains and carrying different genetic modifications are almost invariant in gene expression profile under conditions used to maintain pluripotency. Tissue culture conditions that give the widest range of gene expression and maximise EB growth involve the use of 20% serum and starting cell numbers of 1000 per EB. 23 genes of importance to early development have been identified; more than half of these are also identified using similar studies, thus validating our results. EBs cultured in the same dish vary widely in terms of their gene expression (and hence, undoubtedly, in their future differentiation potential). This may explain some of the inherent variability in differentiation protocols that use EBs. |
| Databáze: | OpenAIRE |
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