Embryo-specific expression of a visual reporter gene as a selection system for citrus transformation

Autor: Leonardo Soriano, Jude W. Grosser, Lígia Erpen, Manjul Dutt, Flavia T. Zambon
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
Citrus
Embryology
Leaves
Agrobacteria
Somatic embryogenesis
lcsh:Medicine
Gene Expression
Plant Science
01 natural sciences
Genetically Modified Plants
Plant Microbiology
Genes
Reporter

Gene expression
lcsh:Science
Regulator gene
Regulation of gene expression
Multidisciplinary
Genetically Modified Organisms
Plant Anatomy
food and beverages
Eukaryota
Plants
Plants
Genetically Modified

Suspension Cultures
Cell biology
Biological Cultures
Genetic Engineering
Research Article
Biotechnology
Transgene
Biology
Research and Analysis Methods
Microbiology
Fruits
03 medical and health sciences
Transformation
Genetic

Genetics
Gene Regulation
Selection
Genetic

Gene
Reporter gene
Bacteria
lcsh:R
fungi
Embryos
Organisms
Biology and Life Sciences
Transformation (genetics)
030104 developmental biology
lcsh:Q
Plant Biotechnology
010606 plant biology & botany
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 1, p e0190413 (2018)
ISSN: 1932-6203
Popis: The embryo-specific Dc3 gene promoter driving the VvMybA1 anthocyanin regulatory gene was used to develop a visual selection system for the genetic transformation of citrus. Agrobacterium-mediated transformation of cell suspension cultures resulted in the production of purple transgenic somatic embryos that could be easily separated from the green non-transgenic embryos. The somatic embryos produced phenotypically normal plants devoid of any visual purple coloration. These results were also confirmed using protoplast transformation. There was minimal gene expression in unstressed one-year-old transgenic lines. Cold and drought stress did not have any effect on gene expression, while exogenous ABA and NaCl application resulted in a minor change in gene expression in several transgenic lines. When gas exchange was measured in intact leaves, the transgenic lines were similar to controls under the same environment. Our results provide conclusive evidence for the utilization of a plant-derived, embryo-specific visual reporter system for the genetic transformation of citrus. Such a system could aid in the development of an all-plant, consumer-friendly GM citrus tree.
Databáze: OpenAIRE