Embryo-specific expression of a visual reporter gene as a selection system for citrus transformation
Autor: | Leonardo Soriano, Jude W. Grosser, Lígia Erpen, Manjul Dutt, Flavia T. Zambon |
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Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
0301 basic medicine Citrus Embryology Leaves Agrobacteria Somatic embryogenesis lcsh:Medicine Gene Expression Plant Science 01 natural sciences Genetically Modified Plants Plant Microbiology Genes Reporter Gene expression lcsh:Science Regulator gene Regulation of gene expression Multidisciplinary Genetically Modified Organisms Plant Anatomy food and beverages Eukaryota Plants Plants Genetically Modified Suspension Cultures Cell biology Biological Cultures Genetic Engineering Research Article Biotechnology Transgene Biology Research and Analysis Methods Microbiology Fruits 03 medical and health sciences Transformation Genetic Genetics Gene Regulation Selection Genetic Gene Reporter gene Bacteria lcsh:R fungi Embryos Organisms Biology and Life Sciences Transformation (genetics) 030104 developmental biology lcsh:Q Plant Biotechnology 010606 plant biology & botany Developmental Biology |
Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 1, p e0190413 (2018) |
ISSN: | 1932-6203 |
Popis: | The embryo-specific Dc3 gene promoter driving the VvMybA1 anthocyanin regulatory gene was used to develop a visual selection system for the genetic transformation of citrus. Agrobacterium-mediated transformation of cell suspension cultures resulted in the production of purple transgenic somatic embryos that could be easily separated from the green non-transgenic embryos. The somatic embryos produced phenotypically normal plants devoid of any visual purple coloration. These results were also confirmed using protoplast transformation. There was minimal gene expression in unstressed one-year-old transgenic lines. Cold and drought stress did not have any effect on gene expression, while exogenous ABA and NaCl application resulted in a minor change in gene expression in several transgenic lines. When gas exchange was measured in intact leaves, the transgenic lines were similar to controls under the same environment. Our results provide conclusive evidence for the utilization of a plant-derived, embryo-specific visual reporter system for the genetic transformation of citrus. Such a system could aid in the development of an all-plant, consumer-friendly GM citrus tree. |
Databáze: | OpenAIRE |
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