Aspalatone Prevents VEGF-Induced Lipid Peroxidation, Migration, Tube Formation, and Dysfunction of Human Aortic Endothelial Cells
Autor: | Kirtikar Shukla, Himangshu Sonowal, Kota V. Ramana, Pabitra Bikash Pal |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Vascular Endothelial Growth Factor A Aging Article Subject Blotting Western Apoptosis Pharmacology Biology Biochemistry Antioxidants Monocytes Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Enos Cell Movement medicine Cell Adhesion Humans Endothelial dysfunction lcsh:QH573-671 Aorta Cells Cultured Cell Proliferation Tube formation Aspirin lcsh:Cytology Leptin Cell migration Cell Biology General Medicine Malondialdehyde medicine.disease biology.organism_classification Pathophysiology 030104 developmental biology chemistry Immunology cardiovascular system Endothelium Vascular Lipid Peroxidation Research Article Signal Transduction |
Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2017 (2017) |
ISSN: | 1942-0994 1942-0900 |
Popis: | Although aspalatone (acetylsalicylic acid maltol ester) is recognized as an antithrombotic agent with antioxidative and antiplatelet potential; its efficacy in preventing endothelial dysfunction is not known. In this study, we examined the antiangiogenic, antioxidative, and anti-inflammatory effect of aspalatone in human aortic endothelial cells (HAECs). Specifically, the effect of aspalatone on VEGF-induced HAECs growth, migration, tube formation, and levels of lipid peroxidation-derived malondialdehyde (MDA) was examined. Our results indicate that the treatment of HAECs with aspalatone decreased VEGF-induced cell migration, tube formation, and levels of MDA. Aspalatone also inhibited VEGF-induced decrease in the expression of eNOS and increase in the expression of iNOS, ICAM-1, and VCAM-1. Aspalatone also prevented the VEGF-induced adhesion of monocytes to endothelial cells. Furthermore, aspalatone also prevented VEGF-induced release of inflammatory markers such as Angiopoietin-2, Leptin, EGF, G-CSF, HB-EGF, and HGF in HAECs. Thus, our results suggest that aspalatone could be used to prevent endothelial dysfunction, an important process in the pathophysiology of cardiovascular diseases. |
Databáze: | OpenAIRE |
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