Fine-needle cytology and flow cytometry assessment of reactive and lymphoproliferative processes of the breast
| Autor: | Elena Vigliar, Immacolata Cozzolino, Albina Riccardi, Leda Di Pietto, Giancarlo Troncone, Pio Zeppa, Laura Virginia Sosa Fernandez, Marco Picardi, Fabrizio Pane, Antonio Vetrani |
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| Přispěvatelé: | Vigliar, E, Cozzolino, I, Fernandez, Lv, Di Pietto, L, Riccardi, A, Picardi, Marco, Pane, Fabrizio, Vetrani, Antonio, Troncone, Giancarlo, Zeppa, P. |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Histology Biopsy Fine-Needle Breast Lymphoma Pathology and Forensic Medicine Flow cytometry Diagnosis Differential Breast Diseases Young Adult Cytology Biopsy medicine Humans Intramammary lymph nodes Lymphocytes Mammary Glands Human breast Aged Aged 80 and over flow cytometry assessment Primary (chemistry) medicine.diagnostic_test business.industry General Medicine Middle Aged medicine.disease Flow Cytometry Lymphoproliferative Disorders Lymphoma lymphoproliferative processes Fine-needle cytology Female business flow cytometry |
| Popis: | Objective: The breast may be affected by reactive and lymphoproliferative processes such as primary (PBL) or secondary (SBL) lymphoma, reactive intramammary lymph nodes and sclerosing lobulitis; imaging may be not specific and surgical treatment not indicated. We report an experience with fine-needle cytology (FNAC) combined with flow cytometry (FC) and immunocytochemistry (ICC) in the diagnosis of these processes. Study Design: Thirty-seven cases comprising intramammary lymph nodes (n = 15), sclerosing lobulitis (n = 2), PBL (n = 11) and SBL (n = 9) are reported. FNAC was used to prepare traditional smears, conventional ICC or FC. Cytological diagnoses were compared to the imaging data, checked by histology or follow-up and statistically evaluated. Results: Imaging was not conclusive in most PBL, SBL, sclerosing lobulitis and some intramammary lymph nodes. FNAC combined with FC and ICC provided a definitive diagnosis of intramammary lymph node, sclerosing lobulitis, PBL and SBL in 18 cases with indication of the specific subtype in 10 cases. Statistical analysis showed 90% sensitivity, 100% specificity, 100% positive predictive value and 89% negative predictive value. Conclusions: FNAC combined with FC and ICC is a helpful procedure for the diagnosis of reactive and lymphoproliferative processes of the breast. It may prevent unnecessary biopsy and speed up therapeutic procedures. |
| Databáze: | OpenAIRE |
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