Evaluation of stability and in vitro wound healing potential of melatonin loaded (lipid enriched) chitosan based microspheres

Autor: Anita Hafner, Marieta Duvnjak Romić, Biserka Cetina-Čižmek, Anja Sušac, Jasmina Lovrić, Jelena Filipović-Grčić
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Pharmaceutical Science
Nanoparticle
Biocompatible Materials
melatonin
wound healing
02 engineering and technology
Cell Line
BIOMEDICINE AND HEALTHCARE. Pharmacy. Pharmacy
Matrix (chemical analysis)
Melatonin
Chitosan
03 medical and health sciences
chemistry.chemical_compound
Drug Delivery Systems
Drug Stability
Zeta potential
medicine
Humans
Particle Size
BIOMEDICINA I ZDRAVSTVO. Farmacija. Farmacija
melatonin
nanoparticles
microspheres
chitosan
stability
wound healing
Pharmaceutical industry
Skin
030304 developmental biology
nanoparticles
microspheres
chitosan
stability
Pharmacology
Drug Carriers
0303 health sciences
Chromatography
General Medicine
Fibroblasts
021001 nanoscience & nanotechnology
Lipids
In vitro
Nanostructures
chemistry
Particle size
HD9665-9675
0210 nano-technology
Wound healing
medicine.drug
Zdroj: Acta Pharmaceutica
Volume 69
Issue 4
Acta Pharmaceutica, Vol 69, Iss 4, Pp 635-648 (2019)
ISSN: 1330-0075
1846-9558
Popis: The aim of this study was to evaluate long-term stability and assess the wound healing potential of the innovative melatonin-loaded lipid-enriched hybrid system compared to conventional melatonin-loaded chitosan microspheres. The hybrid system contained nanostructured lipid carrier incorporated in the chitosan matrix, in order to modify melatonin release and alter physicochemical characteristics of the delivery system. Stability testing was performed during a six-month period under two conditions: refrigerated (5 ± 3 °C) and at room temperature (25 ± 2 °C/60 ± 5 % RH). Samples stored at both conditions were analyzed in terms of particle size, zeta potential, moisture content and thermal properties. At the end of testing, drug content was determined in all samples. Dressings wound healing potential was assessed by in vitro scratch test using human skin fibroblast cell line. Although both systems showed good stability characteristics, the addition of lipids in the system has improved its wound healing potential.
Databáze: OpenAIRE
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