Delivery of a cocktail DNA vaccine encoding cysteine proteinases type I, II and III with solid lipid nanoparticles potentiate protective immunity against Leishmania major infection
| Autor: | Alireza Vatanara, Fatemeh Torkashvand, Rouholah Vahabpour, Sima Rafati, Farnaz Zahedifard, Yasaman Taslimi, Abdolhossein Rouholamini Najafabadi, Behrooz Vaziri, Delaram Doroud |
|---|---|
| Přispěvatelé: | Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP), Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Biotechnology Research Center, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) |
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_treatment
030231 tropical medicine Leishmaniasis Cutaneous Pharmaceutical Science Pharmacology Biology DNA vaccination Interferon-gamma Mice Leishmania Cocktail DNA vaccine 03 medical and health sciences 0302 clinical medicine Immune system Immunity Solid lipid nanoparticle Vaccines DNA medicine Animals Leishmania major Delivery system 030304 developmental biology Mice Inbred BALB C 0303 health sciences Cationic solid lipid nanoparticles Vaccination biology.organism_classification Lipids Cysteine proteinases 3. Good health Cysteine Endopeptidases Cytokine Immunoglobulin G Antibody Formation Immunology Nanoparticles Female [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology Adjuvant |
| Zdroj: | Journal of Controlled Release Journal of Controlled Release, Elsevier, 2011, 153 (3), pp.154-62. ⟨10.1016/j.jconrel.2011.04.011⟩ |
| ISSN: | 0168-3659 |
| Popis: | International audience; Earlier generations of Leishmania vaccines have reached the third-phase of clinical trials, however none of them have shown adequate efficacy due to lack of an appropriate adjuvant. In this study, cationic solid lipid nanoparticles (cSLNs) were used to formulate three pDNAs encoding L. major cysteine proteinase type I (cpa), II (cpb) and III (cpc). BALB/c mice were immunized twice with a 3-week interval, with SLN-pcDNA-cpa/b/c, pcDNA-cpa/b/c, SLN, SLN-pcDNA and PBS. Footpad assessments, parasite burden, cytokine and antibody responses were evaluated. Mice vaccinated with SLN-pcDNA-cpa/b/c significantly (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect