Differential sensitivity of human platelet P2X1 and P2Y1 receptors to disruption of lipid rafts
| Autor: | Catherine Vial, C. Y. Eleanor Fung, Martyn P. Mahaut-Smith, Alison H. Goodall, Richard J. Evans |
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| Rok vydání: | 2006 |
| Předmět: |
Blood Platelets
Membrane Fluidity Biophysics Differential Threshold Gating Biology Biochemistry Receptors Purinergic P2Y1 chemistry.chemical_compound Membrane Microdomains Membrane fluidity Humans Platelet Platelet activation Receptor Molecular Biology Lipid raft Cells Cultured Receptors Purinergic P2 Cholesterol Cell Biology Cell biology chemistry Receptors Purinergic P2X Calcium lipids (amino acids peptides and proteins) Intracellular |
| Zdroj: | Biochemical and Biophysical Research Communications. 343:415-419 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.02.174 |
| Popis: | ATP-stimulated P2X1 and ADP-stimulated P2Y1 receptors play important roles in platelet activation. An increase in intracellular Ca2+ represents a key signalling event coupled to both of these receptors, mediated via direct gating of Ca2+-permeable channels in the case of P2X1 and phospholipase-C-dependent Ca2+ mobilisation for P2Y1. We show that disruption of cholesterol-rich membrane lipid rafts reduces P2X1 receptor-mediated calcium increases by approximately 80%, while P2Y1 receptor-dependent Ca2+ release is unaffected. In contrast to artery, vas deferens, bladder smooth muscle, and recombinant expression in cell lines, where P2X1 receptors show almost exclusive association with lipid rafts, only approximately 20% of platelet P2X1 receptors are co-expressed with the lipid raft marker flotillin-2. We conclude that lipid rafts play a significant role in the regulation of P2X1 but not P2Y1 receptors in human platelets and that a reserve of non-functional P2X1 receptors may exist. |
| Databáze: | OpenAIRE |
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