Release of Mitochondrial and Nuclear DNA During On-Pump Heart Surgery: Kinetics and Relation to Extracellular Vesicles
| Autor: | Michael M. Galagudza, Anna Kostareva, Arno Ruusalepp, S. M. Minasian, Anton Baysa, Guro Valen, Anton Fedorov, K. A. Kondratov, Jarle Vaage, Maxim N. Popov, Dmitry Kurapeev, Alexey Yakovlev, Kåre-Olav Stensløkken |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Mitochondrial DNA medicine.medical_specialty Pharmaceutical Science 030204 cardiovascular system & hematology Exosomes DNA Mitochondrial law.invention 03 medical and health sciences Coronary circulation 0302 clinical medicine law medicine.artery Genetics Extracellular Cardiopulmonary bypass Humans Medicine Coronary Artery Bypass Genetics (clinical) Coronary sinus Cardiopulmonary Bypass business.industry Cardiac surgery Surgery Kinetics surgical procedures operative 030104 developmental biology medicine.anatomical_structure Pulmonary artery Molecular Medicine Cardiology and Cardiovascular Medicine business Cell-Free Nucleic Acids Artery |
| Zdroj: | Journal of Cardiovascular Translational Research. 12:184-192 |
| ISSN: | 1937-5395 1937-5387 |
| Popis: | During heart surgery with cardiopulmonary bypass (CPB), the release of mitochondrial (mtDNA) and nuclear DNA (nDNA) and their association to extracellular vesicles were investigated. In patients undergoing elective coronary artery bypass grafting (CABG, n = 12), blood was sampled before, during, and after surgery from peripheral artery, pulmonary artery, and the coronary sinus. Plasma was separated in three fractions: microvesicles, exosomes, and supernatant. mtDNA and nDNA were measured by qPCR. mtDNA and nDNA levels increased after start of surgery, but before CPB, and increased further during CPB. mtDNA copy number was about 1000-fold higher than nDNA. mtDNA was predominantly localized to the vesicular fractions in plasma, whereas nDNA was predominantly in the supernatant. The amount of free mtDNA increased after surgery. There was no net release or disappearance of DNAs across the pulmonary, systemic, or coronary circulation. Extracellular DNAs, in particular mtDNA, may be important contributors to the whole-body inflammation during CPB. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink