A 50-kilodalton Cry2A peptide is lethal to Bombyx mori and Lymantria dispar

Autor:	Toshiaki Mitsui, Masayuki Taniguchi, Hidetaka Hori, Kohsuke Haginoya, Shin-ichiro Asano, Tomoaki Kouya, Kazuhisa Miyamoto, Miki Tanaka, Mitsuaki Shimazu, Masataka Ohsawa, Kenta Moriyama
Rok vydání:	2012
Předmět:	Bacillus thuringiensis Peptide Applied Microbiology and Biotechnology Microbiology Kilodalton Lepidoptera genitalia Hemolysin Proteins Bacterial Proteins Bombyx mori Lymantria dispar parasitic diseases Invertebrate Microbiology Animals Chymotrypsin chemistry.chemical_classification Larva Ecology biology Bacillus thuringiensis Toxins Hydrolysis fungi biology.organism_classification Molecular biology Survival Analysis Endotoxins Lepidoptera chemistry biology.protein Peptides Food Science Biotechnology
Zdroj:	Applied and environmental microbiology. 78(13)
ISSN:	1098-5336
Popis:	The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis , and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides killed the larvae of both insects.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::666467d9a5c27eb8f6f145af416c4abb https://pubmed.ncbi.nlm.nih.gov/22544240 Zobrazit plný text záznamu