| Autor: |
María Azucena Castañeda-Montes, Julieta Sandra Cuevas-Romero, José Luis Cerriteño-Sánchez, Lucero de María Ávila-De la Vega, José Bryan García-Cambrón, Hugo Ramírez-Álvarez |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Bioscience, Biotechnology, and Biochemistry. 87:267-278 |
| ISSN: |
1347-6947 |
| Popis: |
Small ruminant lentiviruses (SRLV) infect sheep and goats resulting in significant economic losses. This study evaluated for the first time the predicted conformational structure of the SRLV-capsid-protein 25 (SRLV-p25) and analyzed the antigenicity of recombinant protein (SRLV-rp25) in mice by coupling to an immunostimulatory complexes based on glycyrrhizinic acid liposomes (GAL) and tested plasma from goats and sheep naturally infected. Analysis in silico and conformational structure of SRLV-p25 (genotype B-FESC-752) showed similar characteristics to other lentiviral capsids. The efficient expression of SRLV-rp25 was confirmed by Western blot. The humoral immune responses in mice showed an increased level of antibodies from day 21 to 35 of the SRLV-rp25-GAL and SRLV-rp25-ISCOM® groups and the cellular immune response showed no significant difference in IL-10 levels (P >.05), however, a significant difference (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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